Anticorps Monoclonal anti-FH

• Phare
• Validé par KD/KO

FH Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A9C8

N° de cat : 68162-1-PBS

Synonymes

HsFH, Fumarase, EC:4.2.1.2, 1A9C8

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Galerie de données de validation

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  WB analysis using 68162-1-Ig (same clone as 68162-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68162-1-Ig (FH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68162-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68162-1-Ig (same clone as 68162-1-PBS)

  WB result of FH antibody (68162-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FH transfected HepG2 cells. This data was developed using the same antibody clone with 68162-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 68162-1-Ig (same clone as 68162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68162-1-Ig (FH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68162-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 68162-1-Ig (same clone as 68162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68162-1-Ig (FH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68162-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68162-1-Ig (same clone as 68162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68162-1-Ig (FH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68162-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68162-1-PBS cible FH dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FH Protéine recombinante Ag30254
• Nom complet: fumarate hydratase
• Masse moléculaire calculée: 510 aa, 55 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC003108
• Symbole du gène: FH
• Identification du gène (NCBI): 2271
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation.

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