Anticorps Recombinant de lapin anti-FHIT

FHIT Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

240564D9

N° de cat : 83710-1-PBS

Synonymes

AP3Aase, AP3A hydrolase, Adenylylsulfate-ammonia adenylyltransferase, Adenylylsulfatase, Adenosine 5'-monophosphoramidase FHIT

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Galerie de données de validation

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  WB analysis using 83710-1-RR (same clone as 83710-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83710-1-RR (FHIT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83710-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 83710-1-RR (same clone as 83710-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83710-1-RR (FHIT antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83710-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83710-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83710-1-RR against Human FHIT were performed. The affinity constant is 5.01 nM.

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Informations sur le produit

83710-1-PBS cible FHIT dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FHIT Protéine recombinante Ag4181
• Nom complet: fragile histidine triad gene
• Masse moléculaire calculée: 147 aa, 17 kDa
• Poids moléculaire observé: 15-17 kDa
• Numéro d’acquisition GenBank: BC032336
• Symbole du gène: FHIT
• Identification du gène (NCBI): 2272
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The candidate tumor suppressor gene, FHIT(Fragile histidine triad protein), encompasses the common human chromosomal fragile site at 3p14.2, the hereditary renal cancer translocation breakpoint, and cancer cell homozygous deletions. Fhit hydrolyzes dinucleotide 5',5'''-P1,P3-triphosphate in vitro and mutation of a central histidine abolishes hydrolase activity(PMID:9391102). FHIT has a major role in regulating beta-catenin-mediated gene transcription.

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