Anticorps Polyclonal de lapin anti-FHL1

• Phare
• Validé par KD/KO

FHL1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, poisson-zèbre, rat, souris

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(32)

N° de cat : 10991-1-AP

Synonymes

FHL 1, FHL-1, FHL1B, FLH1A, four and a half LIM domains 1

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Galerie de données de validation

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  WB analysis using 10991-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10991-1-AP

  WB result of FHL1 antibody (10991-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and sg-FHL1 transfected HEK-293 cells.

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  WB analysis of mouse colon using 10991-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10991-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10991-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10991-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10991-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 10991-1-AP

  IP result of anti-FHL1 (IP:10991-1-AP, 4ug; Detection:10991-1-AP 1:8000) with mouse skeletal muscle tissue lysate 4000 ug.

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  IHC staining of human ovary tumor using 10991-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10991-1-AP (FHL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary tumor using 10991-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10991-1-AP (FHL1 antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, cellules HEK-293, tissu cérébral humain, tissu de côlon de souris, tissu de muscle squelettique de rat
• Résultats positifs en IP: tissu de muscle squelettique de souris,
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 27 publications below
• IHC: See 10 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

10991-1-AP cible FHL1 dans les applications de WB, IHC, IF, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, poisson-zèbre, rat, souris

• Réactivité: Humain, poisson-zèbre, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FHL1 Protéine recombinante Ag1448
• Nom complet: four and a half LIM domains 1
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC010998
• Symbole du gène: FHL1
• Identification du gène (NCBI): 2273
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Four-and-a-half LIM domains 1 (FHL1), a member of the FHL protein family, which composed of FHL 1, 2, 3, 4 and ACT (activator of CREM), and characterized by an N-terminal half LIM domain followed by four complete LIM domains. FHL1 may contribute to sarcomere synthesis, assembly and biomechanical stress sensing. FHL1 is also a methylation-silenced tumor-suppressor gene on chromosome X in gastrointestinal cancers, which contributes to the formation of an epigenetic field for cancerization. FHL1(10991-1-AP) is highly expressed in skeletal muscle and to a lesser extent in heart, placenta, ovary, prostate, testis, small intestine, colon and spleen. This is a rabbit polyantibody raised against full length of human FHL1.

Publications

Species	Application	Title
human	WB,IHC,CoIP,ChIP	J Clin Invest; Human four-and-a-half LIM family members suppress tumor cell growth through a TGF-beta-like signaling pathway.; Authors - Ding Lihua L ; KD Validated
human	WB	Genes Dev; Browning of human adipocytes requires KLF11 and reprogramming of PPARγ superenhancers.; Authors - Anne Loft ; KD Validated
human	WB,IHC	Nat Commun; A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity.; Authors - Xiaojie Xu ; KD Validated
		J Cell Biol; Src-mediated phosphorylation converts FHL1 from tumor suppressor to tumor promoter.; Authors - Xiang Wang
human	WB,IHC	Int J Cancer; Downregulation and growth inhibitory role of FHL1 in lung cancer.; Authors - Niu Chang C
mouse,human	WB	J Cell Sci; MAL/MRTF-A controls migration of non-invasive cells by upregulation of cytoskeleton-associated proteins.; Authors - Leitner Laura L

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