Anticorps Polyclonal de lapin anti-FHL2

• Phare
• Validé par KD/KO

FHL2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(15)

Avis (1)

N° de cat : 21619-1-AP

Synonymes

AAG11, DRAL, FHL 2, FHL-2, LIM domain protein DRAL

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Galerie de données de validation

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  WB analysis using 21619-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21619-1-AP (FHL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 21619-1-AP

  IP result of anti-FHL2 (IP:21619-1-AP, 4ug; Detection:21619-1-AP 1:1000) with mouse skeletal muscle tissue lysate 2200ug.

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  IHC staining of human cervical cancer using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat ovary using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat ovary using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 21619-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 21619-1-AP (FHL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 21619-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FHL2 antibody (21619-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules hTERT-RPE1, cellules NIH/3T3, cellules U2OS
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu cardiaque de rat, tissu de cancer de la peau humain, tissu ovarien de rat, tissu ovarien de souris, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 4 publications below

Informations sur le produit

21619-1-AP cible FHL2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FHL2 Protéine recombinante Ag1378
• Nom complet: four and a half LIM domains 2
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC014397
• Symbole du gène: FHL2
• Identification du gène (NCBI): 2274
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FHL2(Four and a half LIM domains protein 2), also known as DRAL. It is located in cytoplasm and nucleus. The protein is expressed in skeletal muscle and heart. May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation. Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes (PMID: 28717008). The molecular weight of FHL2 is 32 kDa.

Publications

Species	Application	Title
human	WB	Nat Commun; A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity.; Authors - Xiaojie Xu
human	WB,IF,CoIP	EBioMedicine; Up-regulated FHL2 inhibits ovulation through interacting with androgen receptor and ERK1/2 in polycystic ovary syndrome.; Authors - Ruiqiong Zhou
mouse,human	WB,IHC,IF,CoIP	Cell Death Dis; FHL2 deficiency impairs follicular development and fertility by attenuating EGF/EGFR/YAP signaling in ovarian granulosa cells; Authors - Chen Wang ; KD Validated
mouse	WB,CoIP	iScience; Genetic ablation of diabetes-associated gene Ccdc92 reduces obesity and insulin resistance in mice; Authors - Lu Ren
human	WB	Int J Mol Sci; FHL2 Inhibits SARS-CoV-2 Replication by Enhancing IFN-β Expression through Regulating IRF-3; Authors - Zhiqiang Xu ; KD Validated
human	WB,IHC	J Virol; Structural and Functional Characterization of Host FHL1 Protein Interaction with Hypervariable Domain of Chikungunya Virus nsP3 Protein.; Authors - Tetyana Lukash

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marina (Verified Customer) (03-24-2025)

80 micrograms of cell lysate (U-373 MG cells). Primary antibody dilution 1:1000, overnight incubation at 4 degrees.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioblastoma cell line

FHL2 Antibody Western Blot validation (1:1000 dilution) in Glioblastoma cell line (Cat no:21619-1-AP)

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