Anticorps Recombinant de lapin anti-FHL2

FHL2 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250585E1

N° de cat : 86060-1-PBS

Synonymes

AAG11, DRAL, FHL 2, FHL-2, LIM domain protein DRAL

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Galerie de données de validation

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  WB analysis using 86060-1-RR (same clone as 86060-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86060-1-RR (FHL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86060-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86060-1-RR (same clone as 86060-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FHL2 antibody (86060-1-RR, Clone: 250585E1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86060-1-PBS in a different storage buffer formulation.

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Informations sur le produit

86060-1-PBS cible FHL2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FHL2 Protéine recombinante Ag29655
• Nom complet: four and a half LIM domains 2
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 30-32 kDa
• Numéro d’acquisition GenBank: BC014397
• Symbole du gène: FHL2
• Identification du gène (NCBI): 2274
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FHL2(Four and a half LIM domains protein 2), also known as DRAL. It is located in cytoplasm and nucleus. The protein is expressed in skeletal muscle and heart. May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation. Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes (PMID: 28717008). The molecular weight of FHL2 is 32 kDa.

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