Anticorps Recombinant de lapin anti-FIP1L1

• Phare
• Validé par KD/KO

FIP1L1 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240658C4

N° de cat : 83863-2-RR

Synonymes

240658C4, FIP1, FIP1-like 1 protein, Pre-mRNA 3'-end-processing factor FIP1, RHE

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Galerie de données de validation

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  WB analysis of MCF-7 using 83863-2-RR

  WB result of FIP1L1 antibody (83863-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FIP1L1 transfected MCF-7 cells.

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  WB analysis using 83863-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83863-2-RR (FIP1L1 antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.

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  FC experiment of A549 using 83863-2-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug FIP1L1 Recombinant antibody (83863-2-RR, Clone:240658C4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  FC experiment of U-2 OS using 83863-2-RR

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug FIP1L1 Recombinant antibody (83863-2-RR, Clone:240658C4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83863-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83863-2-RR against Human FIP1L1 were performed. The affinity constant is 10.8 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293T, cellules MCF-7, cellules U-251, tissu de thymus de souris
• Résultats positifs en FC (Intra): cellules A549, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:15000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83863-2-RR cible FIP1L1 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FIP1L1 Protéine recombinante Ag2921
• Nom complet: FIP1 like 1 (S. cerevisiae)
• Masse moléculaire calculée: 594 aa, 66 kDa
• Poids moléculaire observé: 67-80 kDa
• Numéro d’acquisition GenBank: BC017724
• Symbole du gène: FIP1L1
• Identification du gène (NCBI): 81608
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FIP1L1, also known as FIP1, encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that links the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion causes some forms of idiopathic hypereosinophilic syndrome (HES)( PMID: 12660384).

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