Anticorps Polyclonal de lapin anti-FKBP10/FKBP65

• Phare
• Validé par KD/KO

FKBP10/FKBP65 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12172-1-PBS

Synonymes

FKBP10, FKBP65, FKBP 65, FKBP 10, FK506-binding protein 10

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Galerie de données de validation

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  WB analysis using 12172-1-AP (same clone as 12172-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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  WB analysis using 12172-1-AP (same clone as 12172-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12172-1-AP (same clone as 12172-1-PBS)

  WB result of FKBP10/FKBP65 antibody (12172-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP10/FKBP65 transfected HeLa cells. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 12172-1-AP (same clone as 12172-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 12172-1-AP (same clone as 12172-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 12172-1-AP (same clone as 12172-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 12172-1-AP (same clone as 12172-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 12172-1-AP (same clone as 12172-1-PBS)

  IP result of anti-FKBP10/FKBP65 (IP:12172-1-AP, 4ug; Detection:12172-1-AP 1:600) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 12172-1-AP (same clone as 12172-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 12172-1-AP (FKBP10/FKBP65 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 12172-1-AP (same clone as 12172-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 12172-1-AP (FKBP10/FKBP65 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12172-1-PBS cible FKBP10/FKBP65 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FKBP10/FKBP65 Protéine recombinante Ag2814
• Nom complet: FK506 binding protein 10, 65 kDa
• Masse moléculaire calculée: 582 aa, 64 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC016467
• Symbole du gène: FKBP10
• Identification du gène (NCBI): 60681
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FKBP10, also known as FKBP65, is a 65 kDa FK506 binding protein that is a member of the FKBP-type peptidyl-prolyl cis/trans isomerase family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone and binding partner of type I collagen. FKBP65 is expressed in developing tissues and re-expressed in adult tissues following injury. As documented, FKBP10 has been detected to play important roles in the initiation and progression of cancers.

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