Anticorps Polyclonal de lapin anti-FKBP1A

• Phare
• Validé par KD/KO

FKBP1A Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 10273-1-AP

Synonymes

12 kDa FK506-binding protein, Calstabin-1, FK506-binding protein 1A, FKBP 1A, FKBP1

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Galerie de données de validation

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  WB analysis using 10273-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10273-1-AP (FKBP1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10273-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10273-1-AP (FKBP1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10273-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10273-1-AP (FKBP1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 10273-1-AP

  Immunohistochemical analysis of paraffin-embedded normal colon slide using 10273-1-AP (FKBP1A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 10273-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10273-1-AP (FKBP1A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 10273-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10273-1-AP (FKBP1A antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules Jurkat, tissu cérébral de rat
• Résultats positifs en IHC: human normal colon, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below

Informations sur le produit

10273-1-AP cible FKBP1A dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FKBP1A Protéine recombinante Ag0406
• Nom complet: FK506 binding protein 1A, 12kDa
• Masse moléculaire calculée: 12 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC001925
• Symbole du gène: FKBP1A
• Identification du gène (NCBI): 2280
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FKBP1A(FK506-binding protein 1A) is also named as FKBP1, FKBP12 and belongs to the FKBP-type PPIase family.It keeps in an inactive conformation TGFBR1, the TGF-beta type I serine/threonine kinase receptor, preventing TGF-beta receptor activation in absence of ligand and catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

Publications

Species	Application	Title
human	WB	Cell Rep; Sequencing of captive target transcripts identifies the network of regulated genes and functions of primate-specific miR-522.; Authors - Shen Mynn Tan
pig	WB	Food Chem; TMT-based quantitative proteomic analysis of porcine muscle associated with postmortem meat quality.; Authors - Xinhua Hou
mouse	WB,IHC	Cell Biol Toxicol; Andrographolide ameliorates sepsis-induced acute liver injury by attenuating endoplasmic reticulum stress through the FKBP1A-mediated NOTCH1/AK2 pathway; Authors - Jiaqi He
human	WB	Oncotarget; TGFBR-IDH1-Cav1 axis promotes TGF-β signalling in cancer-associated fibroblast.; Authors - Xiaodan Hou
human	WB	Mol Pharmacol; FK506 Binding Protein 12 Modulates μ Opioid Receptor Phosphorylation and PKC{epsilon}-dependent Signaling by its Direct Interaction with the Receptor.; Authors - Qiu Yu Y ; KD Validated
mouse	WB	Development; PFN4 is required for manchette development and acrosome biogenesis during mouse spermiogenesis; Authors - Naila Umer

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