Anticorps Recombinant de lapin anti-FKBP1A

FKBP1A Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250923G12

N° de cat : 86249-1-PBS

Synonymes

12 kDa FK506-binding protein, Calstabin-1, FK506-binding protein 1A, FKBP 1A, FKBP1

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Galerie de données de validation

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  WB analysis using 86249-1-RR (same clone as 86249-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86249-1-RR (FKBP1A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86249-1-PBS in a different storage buffer formulation.

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  WB analysis using 86249-1-RR (same clone as 86249-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86249-1-RR (FKBP1A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86249-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02313-2

  The mean FKBP1A concentration was determined to be 26.2 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02313-2

  Sandwich ELISA standard curve of MP02313-2, Human FKBP1A Recombinant Matched Antibody Pair - PBS only. 86249-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0406. 86249-4-PBS was HRP conjugated as the detection antibody. Range: 31.3-2000 pg/mL.

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  Affinity and Kinetic Characterization of 86249-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86249-1-RR against Human FKBP1A were performed. The affinity constant is 0.899 nM.

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Informations sur le produit

86249-1-PBS cible FKBP1A dans les applications de WB, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FKBP1A Protéine recombinante Ag0406
• Nom complet: FK506 binding protein 1A, 12kDa
• Masse moléculaire calculée: 12 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC001925
• Symbole du gène: FKBP1A
• Identification du gène (NCBI): 2280
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FKBP1A(FK506-binding protein 1A) is also named as FKBP1, FKBP12 and belongs to the FKBP-type PPIase family.It keeps in an inactive conformation TGFBR1, the TGF-beta type I serine/threonine kinase receptor, preventing TGF-beta receptor activation in absence of ligand and catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

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