Anticorps Polyclonal de lapin anti-FKBP3

• Phare
• Validé par KD/KO

FKBP3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 11755-1-AP

Synonymes

FKBP25, FKBP 3, FKBP 25, FK506-binding protein 3, EC:5.2.1.8

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Galerie de données de validation

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  WB analysis of HeLa using 11755-1-AP

  WB result of FKBP3 antibody (11755-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP3 transfected HeLa cells.

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  WB analysis of PC-3 using 11755-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11755-1-AP (FKBP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11755-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11755-1-AP (FKBP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 11755-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11755-1-AP (FKBP3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 11755-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11755-1-AP (FKBP3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 11755-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11755-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11755-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11755-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 11755-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 11755-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11755-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FKBP3 antibody (11755-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HepG2 using 11755-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FKBP3 antibody (11755-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules A431, cellules HeLa
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer du poumon humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

11755-1-AP cible FKBP3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FKBP3 Protéine recombinante Ag2351
• Nom complet: FK506 binding protein 3, 25kDa
• Masse moléculaire calculée: 224 aa, 25 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC020809
• Symbole du gène: FKBP3
• Identification du gène (NCBI): 2287
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	J Cell Mol Med; FKBP3 aggravates the malignant phenotype of diffuse large B-cell lymphoma by PARK7-mediated activation of Wnt/β-catenin signalling; Authors - Xiaojing Xing ; KD Validated
human	IHC	BMC Cancer; Construction of the optimization prognostic model based on differentially expressed immune genes of lung adenocarcinoma.; Authors - Yang Zhai

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