Anticorps Monoclonal anti-FKBP5

FKBP5 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

3B11F3

N° de cat : 67874-1-Ig

Synonymes

FKBP-51, 3B11F3, 51 kDa FK506-binding protein, 54 kDa progesterone receptor-associated immunophilin, AIG6

---

Galerie de données de validation

• 

  WB analysis using 67874-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67874-1-Ig (FKBP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of rat testis using 67874-1-Ig

  rat testis tissue were subjected to SDS PAGE followed by western blot with 67874-1-Ig (FKBP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 67874-1-Ig

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 67874-1-Ig (FKBP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 67874-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67874-1-Ig (FKBP5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67874-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67874-1-Ig (FKBP5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules Jurkat, cellules K-562, cellules LNCaP, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

67874-1-Ig cible FKBP5 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, singe
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FKBP5 Protéine recombinante Ag5514
• Nom complet: FK506 binding protein 5
• Masse moléculaire calculée: 457 aa, 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC042605
• Symbole du gène: FKBP5
• Identification du gène (NCBI): 2289
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FKBP5, also known as FKBP51, belongs to the family of immunophilins, FK506 binding proteins (FKBPs). FKBP5 is a negative regulator of GR activity and plays a key role in the termination of the stress response by GRs. FKBP5 limits GR function by decreasing ligand-binding sensitivity, thereby delaying nuclear translocation and reducing GR-dependent transcriptional activity. The known functions of FKBP5 include the regulation of steroid hormone receptors, inhibiting apoptosis in cancer cells, promoting Akt1 dephosphorylation, and inhibiting the Akt1 pathways, to name a few. FKBP5 is widely expressed and the calculated molecular weight is 51 kDa (PMID: 21119664, PMID: 34077736, PMID: 37154033).

Publications

Species	Application	Title
human	WB,IHC	Stem Cell Res Ther; Single-cell sequencing of facial adipose tissue unveils FKBP5 as a therapeutic target for facial infiltrating lipomatosis; Authors - Hongrui Chen ; KD Validated
monkey	WB,IF	Neurosci Bull; Single-Nucleus Transcriptomics of the Nucleus Accumbens Reveals Cell-Type-Specific Dysregulation in Adolescent Macaques with Depressive-Like Behaviors; Authors - Teng Teng

{{ptg:RelatedPrimaryAntibodies}}