Anticorps Polyclonal de lapin anti-FKBP8

• Phare
• Validé par KD/KO

FKBP8 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11173-1-PBS

Synonymes

FKBP-38, FKBP38, FKBP 8, FKBP 38, FK506-binding protein 8

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Galerie de données de validation

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  WB analysis of HeLa using 11173-1-AP (same clone as 11173-1-PBS)

  WB result of FKBP8 antibody (11173-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP8 transfected HeLa cells. This data was developed using the same antibody clone with 11173-1-PBS in a different storage buffer formulation.

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  WB analysis using 11173-1-AP (same clone as 11173-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11173-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11173-1-AP (same clone as 11173-1-PBS)

  WB result of FKBP8 antibody (11173-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP8 transfected HeLa cells. This data was developed using the same antibody clone with 11173-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 11173-1-AP (same clone as 11173-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11173-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 11173-1-AP (same clone as 11173-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11173-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 11173-1-AP (same clone as 11173-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11173-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse pancreas using 11173-1-AP (same clone as 11173-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11173-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 11173-1-AP (same clone as 11173-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11173-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 11173-1-AP (same clone as 11173-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11173-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 11173-1-AP (same clone as 11173-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11173-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11173-1-AP (same clone as 11173-1-PBS)

  IP result of anti-FKBP8 (IP:11173-1-AP, 4ug; Detection:11173-1-AP 1:500) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 11173-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 11173-1-AP (same clone as 11173-1-PBS)

  Immunofluorescent analysis of SH-SY5Y cells using 11173-1-AP (FKBP8 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11173-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11173-1-PBS cible FKBP8 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FKBP8 Protéine recombinante Ag1649
• Nom complet: FK506 binding protein 8, 38kDa
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC009966
• Symbole du gène: FKBP8
• Identification du gène (NCBI): 23770
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FK506 binding protein 8 (FKBP8), is a member of the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP8 does not seem to have PPIase/rotamase activity, which is different from other members of this family. The active form of FKBP8 may play a role in the regulation of apoptosis. FKBP8 has 3 isoforms produced by alternative splicing.

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