Anticorps Monoclonal anti-FKBP8

• Phare
• Validé par KD/KO

FKBP8 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

1C1G12

N° de cat : 66690-1-Ig

Synonymes

FKBP-38, FKBP38, FKBP 8, FKBP 38, FK506-binding protein 8

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Galerie de données de validation

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  WB analysis of Jurkat using 66690-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66690-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66690-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66690-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66690-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66690-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66690-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human hypothalamus using 66690-1-Ig

  Immunohistochemical analysis of paraffin-embedded human hypothalamus tissue slide using 66690-1-Ig (FKBP8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66690-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66690-1-Ig (FKBP8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 66690-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using FKBP8 antibody (66690-1-Ig, Clone: 1C1G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HeLa, cellules HepG2, cellules NIH/3T3, cellules RAW 264.7
• Résultats positifs en IHC: tissu d'hypothalamus humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

66690-1-Ig cible FKBP8 dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FKBP8 Protéine recombinante Ag1649
• Nom complet: FK506 binding protein 8, 38kDa
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC009966
• Symbole du gène: FKBP8
• Identification du gène (NCBI): 23770
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FK506 binding protein 8 (FKBP8), is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP8 does not seem to have PPIase/rotamase activity, which is different from other members of this family. The active form of FKBP8 may play a role in the regulation of apoptosis. FKBP8 has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB,IF	bioRxiv; Tau phosphorylation suppresses oxidative stress-induced mitophagy via FKBP8 receptor modulation; Authors - Michael O Isei ; KD Validated
mouse	WB	PLoS One; Tau phosphorylation suppresses oxidative stress-induced mitophagy via FKBP8 receptor modulation; Authors - Michael O Isei ; KD Validated
human	CoIP	J Biol Chem; Lysine demethylase KDM1A promotes cell growth via FKBP8-BCL2 axis in hepatocellular carcinoma; Authors - Suli Lv

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