Anticorps Monoclonal anti-FKBP8

• Phare
• Validé par KD/KO

FKBP8 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C1G12

N° de cat : 66690-1-PBS

Synonymes

FKBP-38, FKBP38, FKBP 8, FKBP 38, FK506-binding protein 8

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Galerie de données de validation

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  WB analysis of Jurkat using 66690-1-Ig (same clone as 66690-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66690-1-PBS in a different storage buffer formulation.

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  WB analysis using 66690-1-Ig (same clone as 66690-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66690-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66690-1-Ig (same clone as 66690-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66690-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66690-1-Ig (same clone as 66690-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66690-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66690-1-Ig (same clone as 66690-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66690-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66690-1-Ig (same clone as 66690-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66690-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66690-1-Ig (same clone as 66690-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66690-1-PBS in a different storage buffer formulation.

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  IHC staining of human hypothalamus using 66690-1-Ig (same clone as 66690-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hypothalamus tissue slide using 66690-1-Ig (FKBP8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66690-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66690-1-Ig (same clone as 66690-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66690-1-Ig (FKBP8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66690-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 66690-1-Ig (same clone as 66690-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using FKBP8 antibody (66690-1-Ig, Clone: 1C1G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66690-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66690-1-PBS cible FKBP8 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FKBP8 Protéine recombinante Ag1649
• Nom complet: FK506 binding protein 8, 38kDa
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC009966
• Symbole du gène: FKBP8
• Identification du gène (NCBI): 23770
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FK506 binding protein 8 (FKBP8), is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP8 does not seem to have PPIase/rotamase activity, which is different from other members of this family. The active form of FKBP8 may play a role in the regulation of apoptosis. FKBP8 has 3 isoforms produced by alternative splicing.

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