Anticorps Polyclonal de lapin anti-FLAD1

• Phare
• Validé par KD/KO

FLAD1 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 14118-1-AP

Synonymes

FADS, FAD1, FAD synthase region, FAD synthase, FAD pyrophosphorylase

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Galerie de données de validation

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  WB analysis of HEK-293 using 14118-1-AP

  WB result of FLAD1 antibody (14118-1-AP; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-FLAD1 transfected HEK-293 cells.

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  WB analysis of K-562 using 14118-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14118-1-AP (FLAD1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 14118-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14118-1-AP (FLAD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14118-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14118-1-AP (FADS antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 14118-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 14118-1-AP (FADS antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 14118-1-AP

  IP result of anti-FLAD1 (IP:14118-1-AP, 4ug; Detection:14118-1-AP 1:1000) with K-562 cells lysate 2400 ug.

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  IF Staining of HepG2 using 14118-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FLAD1 antibody (14118-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HEK-293, cellules HepG2, cellules L02, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

14118-1-AP cible FLAD1 dans les applications de WB, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FLAD1 Protéine recombinante Ag5267
• Nom complet: FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae)
• Masse moléculaire calculée: 446 aa, 49 kDa
• Poids moléculaire observé: 50 kDa, 65-70 kDa
• Numéro d’acquisition GenBank: BC011378
• Symbole du gène: FLAD1
• Identification du gène (NCBI): 80308
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FLAD1 is a protein-coding gene for flavin adenine dinucleotide synthetase (FADS), a key enzyme in the FAD biosynthesis process which contains an N-terminal molybdopterin -binding (MPTb) domain and a C-terminal domain (FADS domain) (PMID: 32714079). Alternative splicing of the human FLAD1 gene generates different isoforms of the enzyme FAD synthase. ~60 and ~50 kDa bands correspond to the expected mitochondrial FADS1 and cytosolic FADS2 proteins, respectively (PMID: 29316637).

Publications

Species	Application	Title
human	WB,CoIP	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu
mouse	WB	Ecotoxicol Environ Saf; Di (2-ethylhexyl) phthalate reduces sperm motility by decreasing sperm tail energy supply; Authors - Xiao-Lu Li

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