Anticorps Monoclonal anti-FLAD1

• Phare
• Validé par KD/KO

FLAD1 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F9A8

N° de cat : 68491-1-PBS

Synonymes

FAD1, FAD synthase region, FAD synthase, FAD pyrophosphorylase, EC:2.7.7.2

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Galerie de données de validation

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  WB analysis using 68491-1-Ig (same clone as 68491-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68491-1-Ig (FLAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68491-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 68491-1-Ig (same clone as 68491-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 68491-1-Ig (FLAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68491-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 68491-1-Ig (same clone as 68491-1-PBS)

  WB result of FLAD1 antibody (68491-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FLAD1 transfected HEK-293 cells. This data was developed using the same antibody clone with 68491-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68491-1-PBS cible FLAD1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FLAD1 Protéine recombinante Ag6845
• Nom complet: FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae)
• Masse moléculaire calculée: 446 aa, 49 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC011378
• Symbole du gène: FLAD1
• Identification du gène (NCBI): 80308
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FLAD1 is a protein-coding gene for flavin adenine dinucleotide synthetase (FADS), a key enzyme in the FAD biosynthesis process which contains an N-terminal molybdopterin -binding (MPTb) domain and a C-terminal domain (FADS domain) (PMID: 32714079). Alternative splicing of the human FLAD1 gene generates different isoforms of the enzyme FAD synthase. ~60 and ~50 kDa bands correspond to the expected mitochondrial FADS1 and cytosolic FADS2 proteins, respectively (PMID: 29316637).

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