Anticorps Recombinant de lapin anti-FLI1

FLI1 Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

250294F12

N° de cat : 85931-1-PBS

Synonymes

FLI 1, FLI-1, Friend leukemia virus integration 1, Proto-oncogene Fli-1

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Galerie de données de validation

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  WB analysis using 85931-1-RR (same clone as 85931-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85931-1-RR (FLI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85931-1-PBS in a different storage buffer formulation.

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  Sample test of MP02198-1

  The mean FLI1 concentration was determined to be 303.2 ng/mL in Jurkat cell extract based on a 1.9 mg/mL extract load.

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  IHC staining of mouse lung using 85931-1-RR (same clone as 85931-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 85931-1-RR (FLI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85931-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 85931-1-RR (same clone as 85931-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 85931-1-RR (FLI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85931-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 85931-1-RR (same clone as 85931-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85931-1-RR (FLI1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85931-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 85931-1-RR (same clone as 85931-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85931-1-RR (FLI1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85931-1-PBS in a different storage buffer formulation.

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  IF Staining of HEL using 85931-1-RR (same clone as 85931-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEL cells using FLI1 antibody (85931-1-RR, Clone: 250294F12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85931-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 85931-1-RR (same clone as 85931-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using FLI1 antibody (85931-1-RR, Clone: 250294F12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85931-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02198-1

  Cytometric bead array standard curve of MP02198-1, FLI1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85931-3-PBS. Detection antibody: 85931-1-PBS. Standard: Ag36169. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 85931-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85931-1-RR against Human FLI1 were performed. The affinity constant is 0.314 nM.

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Informations sur le produit

85931-1-PBS cible FLI1 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FLI1 Protéine recombinante Ag36169
• Nom complet: Friend leukemia virus integration 1
• Masse moléculaire calculée: 452 aa, 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC010115
• Symbole du gène: FLI1
• Identification du gène (NCBI): 2313
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Friend leukemia virus integration1 (FLI1) that is also termed EWSR2 encodes a sequence-specific transcriptional activator involved in Ewing's sarcoma (EWS), a malignant small, round, blue cell tumour. It is a rare disease in which cancer cells are found in the bone or in soft tissue. The most common areas in which it occurs are the pelvis, the femur, the humerus, the ribs and clavicle. EWS occurs most frequently in teenagers and young adults. FLI1 protein may act as an aberrant transcription factor, but exact mechanisms of oncogenesis remains unknown.

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