Anticorps Recombinant de lapin anti-FLI1

FLI1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250294F12

N° de cat : 85931-1-RR

Synonymes

FLI 1, FLI-1, Friend leukemia virus integration 1, Proto-oncogene Fli-1

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Galerie de données de validation

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  WB analysis using 85931-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85931-1-RR (FLI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 85931-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 85931-1-RR (FLI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 85931-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 85931-1-RR (FLI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 85931-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85931-1-RR (FLI1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 85931-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85931-1-RR (FLI1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEL using 85931-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HEL cells using FLI1 antibody (85931-1-RR, Clone: 250294F12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of Jurkat using 85931-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using FLI1 antibody (85931-1-RR, Clone: 250294F12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85931-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85931-1-RR against Human FLI1 were performed. The affinity constant is 0.314 nM.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HL-60, cellules RAW 264.7, cellules U-937
• Résultats positifs en IHC: tissu pulmonaire de souris, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: HEL cells, cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85931-1-RR cible FLI1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FLI1 Protéine recombinante Ag36169
• Nom complet: Friend leukemia virus integration 1
• Masse moléculaire calculée: 452 aa, 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC010115
• Symbole du gène: FLI1
• Identification du gène (NCBI): 2313
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Friend leukemia virus integration1 (FLI1) that is also termed EWSR2 encodes a sequence-specific transcriptional activator involved in Ewing's sarcoma (EWS), a malignant small, round, blue cell tumour. It is a rare disease in which cancer cells are found in the bone or in soft tissue. The most common areas in which it occurs are the pelvis, the femur, the humerus, the ribs and clavicle. EWS occurs most frequently in teenagers and young adults. FLI1 protein may act as an aberrant transcription factor, but exact mechanisms of oncogenesis remains unknown.

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