Anticorps Monoclonal anti-FLNA

FLNA Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

CloneNo.

1G4H3

N° de cat : 67133-1-Ig

Synonymes

ABP 280, ABPX, Actin binding protein 280, Alpha filamin, DKFZp434P031, Filamin 1, Filamin A, FLN, FLN A, FLN1, FLNA, FMD, MNS, NHBP, Non muscle filamin, OPD, OPD1, OPD2

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Galerie de données de validation

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  WB analysis using 67133-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67133-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67133-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67133-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 67133-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 67133-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67133-1-Ig (FLNA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67133-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67133-1-Ig (FLNA antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67133-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67133-1-Ig (FLNA antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67133-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FLNA antibody (67133-1-Ig, Clone: 1G4H3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 67133-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FLNA antibody (67133-1-Ig, Clone: 1G4H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules A431, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules MCF-7, cellules U2OS, tissu de muscle squelettique humain
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

67133-1-Ig cible FLNA dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FLNA Protéine recombinante Ag23291
• Nom complet: filamin A, alpha (actin binding protein 280)
• Masse moléculaire calculée: 2647 aa, 280 kDa
• Poids moléculaire observé: 280 kDa
• Numéro d’acquisition GenBank: BC014654
• Symbole du gène: FLNA
• Identification du gène (NCBI): 2316
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FLNA encodes a 280 kDa widely expressed actin binding protein called filamin A which can crosslinks actin filaments and links actin filaments to membrane glycoproteins to form a three-dimensional network (PMID:29931263). And filamin A could interact with many other proteins, involved in receptor activation, cell migration and adhesion, cell proliferation, inflammation and tumorigenesis, studies have been reported that FLNA overexpressed in multiple types of tumors, suggesting FLNA may involved in cancer aggressiveness(PMID:29100390). What's more, FLNA is an causative gene of periventricular nodular heterotopia (PNH) (PMID: 29062687)

Publications

Species	Application	Title
human	WB,IF	Front Cell Dev Biol; NudC L279P Mutation Destabilizes Filamin A by Inhibiting the Hsp90 Chaperoning Pathway and Suppresses Cell Migration.; Authors - Min Liu
human	IF	Hum Mol Genet; Expression and subcellular localization of USH1C/harmonin in human retina provides insights into pathomechanisms and therapy; Authors - Kerstin Nagel-Wolfrum
human	IHC	Mol Med Rep; Filamin A regulates EGFR/ERK/Akt signaling and affects colorectal cancer cell growth and migration.; Authors - Kun Wang
human	WB	Cell Death Dis; NudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90.; Authors - Wenwen Chen
human	IF	J Exp Clin Cancer Res; Focal Adhesion Kinase (FAK)-Hippo/YAP transduction signaling mediates the stimulatory effects exerted by S100A8/A9-RAGE system in triple-negative breast cancer (TNBC).; Authors - Damiano Cosimo Rigiracciolo
human	WB	Cell Death Dis; FRMD3 inhibits the growth and metastasis of breast cancer through the ubiquitination-mediated degradation of vimentin and subsequent impairment of focal adhesion; Authors - Wenjun Shao