Anticorps Monoclonal anti-Filamin A

Filamin A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1G4H3

N° de cat : 67133-1-PBS

Synonymes

FLNA, 1G4H3, ABP-280, Actin-binding protein 280, Alpha filamin

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Galerie de données de validation

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  WB analysis using 67133-1-Ig (same clone as 67133-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67133-1-Ig (Filamin A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  WB analysis using 67133-1-Ig (same clone as 67133-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67133-1-Ig (Filamin A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67133-1-Ig (same clone as 67133-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67133-1-Ig (Filamin A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 67133-1-Ig (same clone as 67133-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67133-1-Ig (Filamin A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67133-1-Ig (same clone as 67133-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67133-1-Ig (Filamin A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67133-1-Ig (same clone as 67133-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67133-1-Ig (Filamin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67133-1-Ig (same clone as 67133-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67133-1-Ig (Filamin A antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67133-1-Ig (same clone as 67133-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67133-1-Ig (Filamin A antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67133-1-Ig (same clone as 67133-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Filamin A antibody (67133-1-Ig, Clone: 1G4H3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67133-1-Ig (same clone as 67133-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Filamin A antibody (67133-1-Ig, Clone: 1G4H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67133-1-PBS cible Filamin A dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Filamin A Protéine recombinante Ag23291
• Nom complet: filamin A, alpha (actin binding protein 280)
• Masse moléculaire calculée: 2647 aa, 280 kDa
• Poids moléculaire observé: 280 kDa
• Numéro d’acquisition GenBank: BC014654
• Symbole du gène: FLNA
• Identification du gène (NCBI): 2316
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FLNA encodes a 280 kDa widely expressed actin binding protein called filamin A which can crosslinks actin filaments and links actin filaments to membrane glycoproteins to form a three-dimensional network (PMID:29931263). And filamin A could interact with many other proteins, involved in receptor activation, cell migration and adhesion, cell proliferation, inflammation and tumorigenesis, studies have been reported that FLNA overexpressed in multiple types of tumors, suggesting FLNA may involved in cancer aggressiveness(PMID:29100390). What's more, FLNA is an causative gene of periventricular nodular heterotopia (PNH) (PMID: 29062687)

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