Anticorps Polyclonal de lapin anti-Flotillin 1

• Phare
• Validé par KD/KO

Flotillin 1 Polyclonal Antibody for FC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

41

Avis (1)

N° de cat : 15571-1-AP

Synonymes

FLOT1, flotillin 1

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Galerie de données de validation

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  WB analysis using 15571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15571-1-AP (Flotillin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15571-1-AP (Flotillin 1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15571-1-AP (Flotillin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15571-1-AP

  IP Result of anti-Flotillin 1 (IP:15571-1-AP, 4ug; Detection:15571-1-AP 1:500) with mouse brain tissue lysate 4400ug.

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  IF Staining of HeLa using 15571-1-AP

  Immunofluorescent analysis of Hela cells, using FLOT1 antibody 15571-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 15571-1-AP

  1X10^6 HeLa cells were stained with 0.05ug Flotillin 1 antibody (15571-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-Goat anti-Rabbit IgG with dilution 1:100.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules MG U-87, cellules U-251, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC): FC : 0.05 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 40 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Informations sur le produit

15571-1-AP cible Flotillin 1 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Flotillin 1 Protéine recombinante Ag7902
• Nom complet: flotillin 1
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC001146
• Symbole du gène: FLOT1
• Identification du gène (NCBI): 10211
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.

Publications

Species	Application	Title
human	WB	J Extracell Vesicles; Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.; Authors - Xiaohan Gao
human	WB	Brain; Extracellular vesicle biomarkers for complement dysfunction in schizophrenia; Authors - Ting Xue
mouse	WB	Cell Res; Translocation of mixed lineage kinase domain-like protein to plasma membrane leads to necrotic cell death.; Authors - Xin Chen
human,mouse	WB,IHC	Int J Biol Sci; FLOT1 promotes gastric cancer progression and metastasis through BCAR1/ERK signaling; Authors - Ran Wang ; KD Validated
rat	WB	Cell Death Dis; Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats; Authors - Yajie Wang
human	WB	Mol Ther; EVs Containing Host Restriction Factor IFITM3 Inhibited ZIKV Infection of Fetuses in Pregnant Mice through Trans-placenta Delivery.; Authors - Xue Zou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Guorong (Verified Customer) (03-22-2022)

Good performance with a specific band of expected size and 2 bigger and weaker bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: ARPE-19

Flotillin 1 Antibody Western Blot validation (1:2500 dilution) in ARPE-19 (Cat no:15571-1-AP)

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