Anticorps Polyclonal de lapin anti-VEGFR-1/FLT-1

VEGFR-1/FLT-1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

31

N° de cat : 13687-1-AP

Synonymes

FLT, FLT 1, FLT1, FLT-1, Fms like tyrosine kinase 1, FRT, sflt1, Tyrosine protein kinase FRT, VEGF receptor 1, VEGFR 1, VEGFR1, VEGFR-1, VEGFR-1/FLT-1

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Galerie de données de validation

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  WB analysis of HEK-293 using 13687-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 13687-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 13687-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 13687-1-AP

  IP Result of anti-VEGFR-1/FLT-1 (IP:13687-1-AP, 5ug; Detection:13687-1-AP 1:500) with A549 cells lysate 3300ug.

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  IHC staining of human renal cell carcinoma using 13687-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 13687-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 13687-1-AP

  Immunofluorescent analysis of HeLa cells using 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HUVEC using 13687-1-AP

  1X10^6 HUVEC cells were stained with 0.2ug VEGFR-1/FLT-1 antibody (13687-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu placentaire humain, tissu pulmonaire de souris
• Résultats positifs en IP: cellules A549
• Résultats positifs en IHC: tissu de carcinome à cellules rénales humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HUVEC

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 26 publications below
• IHC: See 7 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• FC: See 1 publications below

Informations sur le produit

13687-1-AP cible VEGFR-1/FLT-1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VEGFR-1/FLT-1 Protéine recombinante Ag4558
• Nom complet: fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
• Masse moléculaire calculée: 1338 aa, 151 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC039007
• Symbole du gène: FLT1
• Identification du gène (NCBI): 2321
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vascular endothelial growth factor receptor-1 (VEGFR-1, FLT-1) is a receptor tyrosine kinase belonging to the VEGFR family. VEGF is a key regulator of physiological angiogenesis and has also been implicated in pathological angiogenesis associated with tumors, intraocular neovascular disorders and other conditions. The biological effects of VEGF are mediated by VEGFR-1 and VEGFR-2. Both the two receptors have seven immunoglobulin-like repeats in the extracellular domain, a single transmembrane region and a tyrosine kinase domain. VEGFR-1 binds VEGFA, PIGF and VEGFB, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Two isoforms of VEGFR-1 exist, a full-length transmembrane form and a short souble form (sVEGFR-1) consisting of only the extracellular ligand-binding domain. (PMID: 12778165; 17109193; 8806634)

Publications

Species	Application	Title
rat	WB	ACS Appl Mater Interfaces; Biocompatible Chitin Hydrogel Incorporated with PEDOT Nanoparticles for Peripheral Nerve Repair.; Authors - Lin Huang
human,mouse	WB,IHC	Biomed Pharmacother; Lychee seed polyphenol ameliorates DR via inhibiting inflammasome/apoptosis and angiogenesis in hRECs and db/db mice; Authors - Xiao-Hong Xiang
human	WB	Stem Cells; Heparanase released from mesenchymal stem cells activates integrin beta1/HIF-2alpha/Flk-1 signaling and promotes endothelial cell migration and angiogenesis.; Authors - Xinyang Hu
mouse	WB	Clin Sci (Lond); Anti-anemia drug FG4592 retards the AKI-to-CKD transition by improving vascular regeneration and antioxidative capability.; Authors - Mengqiu Wu
human	WB	Stem Cell Res Ther; Human endometrium-derived stem cell improves cardiac function after myocardial ischemic injury by enhancing angiogenesis and myocardial metabolism.; Authors - Xuemei Fan
human	WB, IF, IHC	Front Oncol; IL-1β Promotes Vasculogenic Mimicry of Breast Cancer Cells Through p38/MAPK and PI3K/Akt Signaling Pathways.; Authors - Muhammad Azhar Nisar

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