Anticorps Polyclonal de lapin anti-VEGFR3

VEGFR3 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

Avis (1)

N° de cat : 20712-1-AP

Synonymes

FLT 4, FLT4, FLT41, Fms like tyrosine kinase 4, fms related tyrosine kinase 4, LMPH1A, PCL, VEGFR 3, VEGFR3

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Galerie de données de validation

• 

  WB analysis of mouse spleen using 20712-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 20712-1-AP (VEGFR3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20712-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20712-1-AP (VEGFR3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 20712-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20712-1-AP (VEGFR3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 20712-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20712-1-AP (VEGFR3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu splénique de souris, cellules HeLa, cellules PC-3, tissu cardiaque de souris

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

20712-1-AP cible VEGFR3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: fms-related tyrosine kinase 4
• Masse moléculaire calculée: 153 kDa
• Poids moléculaire observé: 125 kDa
• Numéro d’acquisition GenBank: NM_002020
• Symbole du gène: FLT4
• Identification du gène (NCBI): 2324
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FLT4, also named VEGFR-3, belongs to the protein kinase superfamily, Tyr protein kinase family, and CSF-1/PDGF receptor subfamily. It is a receptor for VEGFC. FLT4 has a tyrosine-protein kinase activity. Defects in FLT4 are the cause of lymphedema hereditary type 1 (LYH1A). Defects in FLT4 are found in juvenile hemangioma. The antibody is specific to FLT4.

Publications

Species	Application	Title
human	IF	Mol Cancer; m6A methylation reader IGF2BP2 activates endothelial cells to promote angiogenesis and metastasis of lung adenocarcinoma; Authors - Han Fang
human	IHC	Front Oncol; The Specifically Androgen-Regulated Gene (SARG) Promotes Papillary Thyroid Carcinoma (PTC) Lymphatic Metastasis Through Vascular Endothelial Growth Factor C (VEGF-C) and VEGF Receptor 3 (VEGFR-3) Axis.; Authors - Shuai-Jun Xu
Deer	WB	Stem Cell Res Ther; Deer thymosin beta 10 functions as a novel factor for angiogenesis and chondrogenesis during antler growth and regeneration.; Authors - Wei Zhang
human	WB	Pharmaceuticals (Basel); Use of Early Donated COVID-19 Convalescent Plasma Is Optimal to Preserve the Integrity of Lymphatic Endothelial Cells.; Authors - Nada Amri
human	WB	Mol Cell Endocrinol; Long noncoding RNA GAS5 accelerates diabetic wound healing and promotes lymphangiogenesis via miR-217/Prox1 axis.; Authors - Zhi-You He
human		Acta Pharm; Inhibitory effect of taspine derivative TAD1822-7 on tumor cell growth and angiogenesis via suppression of EphrinB2 and related signaling pathways.; Authors - Rui Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shinford (Verified Customer) (12-15-2023)

We used the concentration of 1:500 to get a very strong band. It is a big volume set.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Lung cancer cell lines, lung cancer tissues

VEGFR3 Antibody Western Blot validation (1:500 dilution) in Lung cancer cell lines, lung cancer tissues (Cat no:20712-1-AP)

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