Anticorps Polyclonal de lapin anti-FMNL1

• Phare
• Validé par KD/KO

FMNL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 27834-1-AP

Synonymes

CLL-associated antigen KW-13, FMNL, Formin-like protein 1

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Galerie de données de validation

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  WB analysis using 27834-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27834-1-AP (FMNL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 27834-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27834-1-AP (FMNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of Caki-1 using 27834-1-AP

  IP result of anti-FMNL1 (IP:27834-1-AP, 2ug; Detection:27834-1-AP 1:2000) with Caki-1 cells lysate 1000 ug. The result was provided by Keli Chai, Xuzhou Medical University.

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  IHC staining of human lymphoma using 27834-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27834-1-AP (FMNL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 27834-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27834-1-AP (FMNL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 27834-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 27834-1-AP (FMNL1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules HeLa, cellules Jurkat
• Résultats positifs en IP: Caki-1,
• Résultats positifs en IHC: tissu de lymphome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

27834-1-AP cible FMNL1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FMNL1 Protéine recombinante Ag27312
• Nom complet: formin-like 1
• Poids moléculaire observé: 125-150 kDa
• Numéro d’acquisition GenBank: BC111564
• Symbole du gène: FMNL1
• Identification du gène (NCBI): 752
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FMNL1,also named as C17orf1, C17orf1B and FMNL, belongs to the formin homology family. It may play a role in the control of cell motility and survival of macrophages. FNMLl protein may be involved in anti-apoptosis mechanisms. It is overexpressed in lymphoid malignances and is found associated with Akt. 27834-1-AP is a rabbit polyclonal antibody recognises the 125-150 kDa protein.(PMID:16494944)

Publications

Species	Application	Title
human	WB	Theranostics; Stiff matrix induced srGAP2 tension gradients control migration direction in triple-negative breast cancer; Authors - Chen Li ; KD Validated
human	WB	Dev Cell; Vimentin intermediate filaments coordinate actin stress fibers and podosomes to determine the extracellular matrix degradation by macrophages; Authors - Xinyi Huang
human	IHC	Gastric Cancer; FHOD1 and FMNL1 formin proteins in intestinal gastric cancer: correlation with tumor-infiltrating T lymphocytes and molecular subtypes.; Authors - Naziha Mansuri
human	WB	Cell Rep; Substrate rigidity dictates colorectal tumorigenic cell stemness and metastasis via CRAD-dependent mechanotransduction.; Authors - Yuhan Chang
human	IHC	Biomed Res Int; Systematic Characterization of Expression Patterns and Immunocorrelations of Formin-Like Genes in Breast Cancer; Authors - Erli Gao
mouse	IHC	Clin Immunol; Formin protein DIAPH1 positively regulates PD-L1 expression and predicts the therapeutic response to anti-PD-1/PD-L1 immunotherapy; Authors - Jie Mei

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