Anticorps Monoclonal anti-FMNL1

FMNL1 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1B12A4

N° de cat : 67587-1-Ig

Synonymes

1B12A4, CLL-associated antigen KW-13, FMNL, Formin-like protein 1

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Galerie de données de validation

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  WB analysis using 67587-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67587-1-Ig (FMNL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67587-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67587-1-Ig (FMNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67587-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67587-1-Ig (FMNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NK-92 using 67587-1-Ig

  NK-92 cells were subjected to SDS PAGE followed by western blot with 67587-1-Ig (FMNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 67587-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using FMNL1 antibody (67587-1-Ig, Clone: 1B12A4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HL-60, cellules K-562, tissu de thymus de porc
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67587-1-Ig cible FMNL1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FMNL1 Protéine recombinante Ag27322
• Nom complet: formin-like 1
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: BC111564
• Symbole du gène: FMNL1
• Identification du gène (NCBI): 752
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FMNL1,also named as C17orf1, C17orf1B and FMNL, belongs to the formin homology family. It may play a role in the control of cell motility and survival of macrophages. FNMLl protein may be involved in anti-apoptosis mechanisms. It is overexpressed in lymphoid malignances and is found associated with Akt. 67587-1-Ig antibody detects the protein around 140-150 kDa which is similar to the results reported in publications.(PMID: 21868368, 22986496 , 23182705)

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