Anticorps Monoclonal anti-FMO4

FMO4 Monoclonal Antibody for WB, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D12E10

N° de cat : 67881-1-PBS

Synonymes

Hepatic flavin-containing monooxygenase 4, FMO2, EC:1.14.13.8, Dimethylaniline monooxygenase [N-oxide-forming] 4, 2D12E10

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Galerie de données de validation

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  WB analysis using 67881-1-Ig (same clone as 67881-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.

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  WB analysis of HuH-7 using 67881-1-Ig (same clone as 67881-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 67881-1-Ig (same clone as 67881-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 67881-1-Ig (same clone as 67881-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.

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  FC experiment of HuH-7 using 67881-1-Ig (same clone as 67881-1-PBS)

  1X10^6 HuH-7 cells were intracellularly stained with 0.4 ug Anti-Human FMO4 (67881-1-Ig, Clone:2D12E10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67881-1-PBS cible FMO4 dans les applications de WB, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FMO4 Protéine recombinante Ag7935
• Nom complet: flavin containing monooxygenase 4
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 63 kDa
• Numéro d’acquisition GenBank: BC002780
• Symbole du gène: FMO4
• Identification du gène (NCBI): 2329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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