Anticorps Polyclonal de lapin anti-FMO5

• Phare
• Validé par KD/KO

FMO5 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 13699-1-AP

Synonymes

Dimethylaniline oxidase 5, FMO 5, FMO5

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Galerie de données de validation

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  WB analysis of human liver using 13699-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13699-1-AP (FMO5 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 13699-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13699-1-AP (FMO5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 13699-1-AP

  IP result of anti-FMO5 (IP:13699-1-AP, 4ug; Detection:13699-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of human breast cancer using 13699-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13699-1-AP (FMO5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13699-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13699-1-AP (FMO5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu hépatique humain, tissu pulmonaire de souris
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below

Informations sur le produit

13699-1-AP cible FMO5 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FMO5 Protéine recombinante Ag4604
• Nom complet: flavin containing monooxygenase 5
• Masse moléculaire calculée: 533 aa, 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC035687
• Symbole du gène: FMO5
• Identification du gène (NCBI): 2330
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Microsomal flavin-containing monooxygenases (FMOs) [dimethylaniline monooxygenase (N-oxide forming) catalyze the FAD-, NADPH- and O2-dependent oxidation of a large number of structurally diverse compounds, including drugs, pesticides, and industrial chemicals containing a soft nucleophile(PMID:12488558 ). FMO5, which belongs to the FMO family, is a lesser component of human liver microsomes and is present at about one-third the level of FMO3. FMO5 protein is also present at very low levels in kidney, however, FMO5 exhibits a severely restricted substrate specificity for most drugs and other xenobiotics examined to date(PMID:10950857). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; Flavin-Containing Monooxygenases Are Conserved Regulators of Stress Resistance and Metabolism.; Authors - Shijiao Huang
mouse	WB	Biochem Pharmacol; The phenotype of a knockout mouse identifies flavin-containing monooxygenase 5 (FMO5) as a regulator of metabolic ageing.; Authors - Sandra G Gonzalez Malagon ; KO Validated
mouse	WB	Drug Metab Dispos; The Molecular Mechanism Regulating Diurnal Rhythm of Flavin-Containing Monooxygenase 5 in Mouse Liver.; Authors - Min Chen
mouse	WB	Front Pharmacol; Puerarin ameliorates non-alcoholic fatty liver disease by inhibiting lipid metabolism through FMO5; Authors - Zhaoyi Li

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