Anticorps Polyclonal de lapin anti-Fibromodulin

Fibromodulin Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 13281-1-AP

Synonymes

FMOD, Collagen-binding 59 kDa protein, FM, Keratan sulfate proteoglycan fibromodulin, KSPG fibromodulin

---

Galerie de données de validation

• 

  WB analysis of human liver using 13281-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13281-1-AP (Fibromodulin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 13281-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Fibromodulin antibody (13281-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Applications testées

• Résultats positifs en WB: tissu hépatique humain
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

13281-1-AP cible Fibromodulin dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Fibromodulin Protéine recombinante Ag4030
• Nom complet: fibromodulin
• Masse moléculaire calculée: 376 aa, 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC035281
• Symbole du gène: Fibromodulin
• Identification du gène (NCBI): 2331
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fibromodulin (FMOD), an extracellular matrix protein, belongs to the small leucine-rich proteoglycan family. Fibromodulin is a collagen-binding protein present in many types of connective tissues, e.g. cartilage, tendon, skin, sclera and cornea (PMID: 2531085). It is involved in fibrillogenesis, cell adhesion, and cytokine activity modulation (PMID: 15741214). Fibromodulin may participate in the assembly of the extracellular matrix as it interacts with type I and type II collagen fibrils and inhibits fibrillogenesis in vitro. It may also regulate TGF-beta activities by sequestering TGF-beta into the extracellular matrix. The calculated molecular mass of fibromodulin is 43 kDa, extracellular fibromodulin appears as one single band of 59 kDa due to glycosylation (PMID: 2531085; 22138190).

Publications

Species	Application	Title
human	WB	iScience; Fibromodulin overexpression drives oral squamous cell carcinoma via activating downstream EGFR signaling; Authors - Lingyun Xia
mouse	WB	J Gerontol A Biol Sci Med Sci; Sex Differences in Response to Diet Enriched with Glutathione Precursors in the Aging Heart; Authors - Aude Angelini
rat	WB	FEBS Open Bio; The role of fibromodulin in myocardial fibrosis in a diabetic cardiomyopathy rat model; Authors - Xiyan Dai

{{ptg:RelatedPrimaryAntibodies}}