Anticorps Monoclonal anti-FMR1

• Phare
• Validé par KD/KO

FMR1 Monoclonal Antibody for Indirect ELISA, IP, IHC, WB

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

Indirect ELISA, IP, IHC, WB

Conjugaison

Non conjugué

CloneNo.

2B5B5

N° de cat : 66548-1-PBS

Synonymes

FRAXA, Fragile X messenger ribonucleoprotein 1, Fragile X messenger ribonucleoprotein, fragile X mental retardation 1, FMRP

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Galerie de données de validation

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  WB analysis using 66548-1-Ig (same clone as 66548-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66548-1-Ig (FMR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 66548-1-Ig (same clone as 66548-1-PBS)

  IP result of anti-FMR1 (IP:66548-1-Ig, 5ug; Detection:66548-1-Ig 1:3000) with HeLa cells lysate 3200 ug. This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66548-1-Ig (same clone as 66548-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66548-1-Ig (same clone as 66548-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66548-1-Ig (same clone as 66548-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66548-1-Ig (same clone as 66548-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66548-1-Ig (same clone as 66548-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66548-1-Ig (same clone as 66548-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66548-1-Ig (same clone as 66548-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 66548-1-Ig (same clone as 66548-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66548-1-Ig (same clone as 66548-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66548-1-Ig (same clone as 66548-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 66548-1-Ig (same clone as 66548-1-PBS)

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human FMR1 (66548-1-Ig, Clone:2B5B5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 66548-1-Ig (same clone as 66548-1-PBS)

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human FMR1 (66548-1-Ig, Clone:2B5B5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66548-1-PBS cible FMR1 dans les applications de Indirect ELISA, IP, IHC, WB et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FMR1 Protéine recombinante Ag4697
• Nom complet: fragile X mental retardation 1
• Masse moléculaire calculée: 632 aa, 71 kDa
• Poids moléculaire observé: 69-72 kDa
• Numéro d’acquisition GenBank: BC038998
• Symbole du gène: FMR1
• Identification du gène (NCBI): 2332
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.

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