Anticorps Monoclonal anti-Fibronectin

• Phare
• Validé par KD/KO

Fibronectin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Cell treatment

Conjugaison

Non conjugué

Publications(152)

CloneNo.

1G10F9

Avis (4)

N° de cat : 66042-1-Ig

Synonymes

FN1, 1G10F9, Anastellin, CIG, Cold insoluble globulin

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Galerie de données de validation

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  WB analysis of human plasma using 66042-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66042-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 66042-1-Ig

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 66042-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MG-63 using 66042-1-Ig

  MG-63 cells were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat serum using 66042-1-Ig

  rat serum were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human plasma using 66042-1-Ig

  IP result of anti-Fibronectin (IP:66042-1-Ig, 5ug; Detection:66042-1-Ig 1:500) with human plasma lysate 4000ug.

• 

  IHC staining of human colon cancer using 66042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon cancer using 66042-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Fibronectin antibody (66042-1-Ig, Clone: 1G10F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human colon cancer using 66042-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Fibronectin antibody (66042-1-Ig, Clone: 1G10F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of NIH/3T3 using 66042-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Fibronectin antibody (66042-1-Ig, Clone: 1G10F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of NIH/3T3 using 66042-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol ) fixed NIH/3T3 cells using 66042-1-Ig(Fibronectin antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu plasmatique humain, cellules HepG2, cellules HuH-7, cellules U2OS, plasma humain, sérum de rat
• Résultats positifs en IP: tissu plasmatique humain
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du côlon humain,
• Résultats positifs en IF/ICC: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 97 publications below
• IHC: See 44 publications below
• IF: See 44 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

66042-1-Ig cible Fibronectin dans les applications de WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Cell treatment et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Fibronectin Protéine recombinante Ag8016
• Nom complet: fibronectin 1
• Masse moléculaire calculée: 2386 aa, 263 kDa
• Poids moléculaire observé: 263 kDa
• Numéro d’acquisition GenBank: BC005858
• Symbole du gène: Fibronectin
• Identification du gène (NCBI): 2335
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)

Publications

Species	Application	Title
mouse	WB	Protein Cell; Neutrophil extracellular traps license macrophage production of chemokines to facilitate CD8+ T cell infiltration in obstruction-induced renal fibrosis; Authors - Hongshuai Jia
human	IF	Dev Cell; Vimentin intermediate filaments coordinate actin stress fibers and podosomes to determine the extracellular matrix degradation by macrophages; Authors - Xinyi Huang
rat	WB,IF	Int J Biol Macromol; A multifunctional polydopamine/genipin/alendronate nanoparticle licences fibrin hydrogels osteoinductive and immunomodulatory potencies for repairing bone defects; Authors - Wentao Shi
human	WB,IF	Biomed Pharmacother; Nintedanib prevents TGF-β2-induced epithelial-mesenchymal transition in retinal pigment epithelial cells; Authors - Yiwei Yin
mouse	IHC	Oxid Med Cell Longev; HBO1 as an Important Target for the Treatment of CCL4-Induced Liver Fibrosis and Aged-Related Liver Aging and Fibrosis; Authors - Baopeng Xing
mouse	IHC	Front Immunol; Integrated analysis reveals crosstalk between pyroptosis and immune regulation in renal fibrosis; Authors - Fengxia Bai

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (04-19-2023)

Worked well for IP, band seen just below 250 kD (page ruler plus ladder). Lower band is light chain IgG

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: BM-MSCs

Fibronectin Antibody Western Blot,Immunoprecipitation validation (1:1000 dilution) in BM-MSCs (Cat no:66042-1-Ig)

FH

Ren Jie (Verified Customer) (07-11-2022)

Quite significant smear and non-specific binding

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human imortalized mammary fibroblast

Fibronectin Antibody Western Blot validation (1:1000 dilution) in Human imortalized mammary fibroblast (Cat no:66042-1-Ig)

FH

Deqiang (Verified Customer) (05-14-2020)

it works well on mouse embryo paraffin sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100

FH

Jihyun (Verified Customer) (05-07-2020)

There is no background signal. I can get a good one band in the WB. Great!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse epithelial cell

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