Anticorps Polyclonal de lapin anti-FNDC3B

• Phare
• Validé par KD/KO

FNDC3B Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 22605-1-AP

Synonymes

FAD104, fibronectin type III domain containing 3B, Fibronectin type III domain-containing protein 3B, HCV NS5A-binding protein 37, UNQ2421/PRO4979/PRO34274

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Galerie de données de validation

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  WB analysis of 4T1 using 22605-1-AP

  4T1 cells were subjected to SDS PAGE followed by western blot with 22605-1-AP (FNDC3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 22605-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 22605-1-AP (FNDC3B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 22605-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22605-1-AP (FNDC3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 22605-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 22605-1-AP (FNDC3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 22605-1-AP

  IP result of anti-FNDC3B (IP:22605-1-AP, 4ug; Detection:22605-1-AP 1:1000) with HepG2 cells lysate 2400ug.

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  IHC staining of human liver using 22605-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22605-1-AP (FNDC3B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 22605-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22605-1-AP (FNDC3B Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules 4T1, cellules C6, cellules HepG2, cellules HL-60
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

22605-1-AP cible FNDC3B dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FNDC3B Protéine recombinante Ag18357
• Nom complet: fibronectin type III domain containing 3B
• Masse moléculaire calculée: 1204 aa, 133 kDa
• Poids moléculaire observé: 150 kDa, 70 kDa
• Numéro d’acquisition GenBank: BC039297
• Symbole du gène: FNDC3B
• Identification du gène (NCBI): 64778
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fibronectin type III domain-containing protein 3B(FNDC3B), also named FAD104, belongs to the FNDC3 family. NDC3B is a positive regulator of adipocyte differentiation and is expressed at an early stage of adipocyte differentiation. FNDC3B is an endoplasmic reticulum transmembrane protein with a single transmembrane domain at the C terminus preceded by nine repeated fibronectin type III domains(PMID: 32966780). Due to the ability of the fibronectin type III domain to bind to a variety of proteins, FNDC3B plays a crucial role in cell adhesion, proliferation, and growth signaling. FNDC3B is abnormally expressed in a variety of human cancers, including hepatocellular carcinoma, acute myeloid leukemia, colorectal carcinoma, and cervical carcinoma(PMID: 36275754).

Publications

Species	Application	Title
human	WB,IHC,IF	Cancer Sci; FNDC3B 3'-UTR shortening escapes from microRNA-mediated gene repression and promotes nasopharyngeal carcinoma progression.; Authors - Ying-Qing Li ; KD Validated
human	WB	Pediatr Res; Hirschsprung's disease: key microRNAs and target genes.; Authors - Mei Hong
human	WB	Cancers (Basel); Integrated Analysis of RNA-Binding Proteins in Glioma.; Authors - Zhixing Wang
human	WB	J Cell Physiol; FNDC3B circular RNA promotes the migration and invasion of gastric cancer cells via the regulation of E-cadherin and CD44 expression.; Authors - Yuling Hong
human	WB,IHC	Am J Transl Res; EYA1 promotes cell migration and tumor metastasis in hepatocellular carcinoma.; Authors - Deguang Kong
human	WB	J Cell Commun Signal; CircRNA hsa_circ_0001627 aggravates cervical cancer progression through upregulation of FNDC3B and activating PI3K/mTOR signaling pathway; Authors - Yan Li

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