Anticorps Recombinant de lapin anti-FNDC3B

FNDC3B Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250827H1

N° de cat : 86272-1-PBS

Synonymes

FAD104, fibronectin type III domain containing 3B, Fibronectin type III domain-containing protein 3B, HCV NS5A-binding protein 37, UNQ2421/PRO4979/PRO34274

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Galerie de données de validation

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  WB analysis using 86272-1-RR (same clone as 86272-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86272-1-RR (FNDC3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86272-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 86272-1-RR (same clone as 86272-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86272-1-RR (FNDC3B antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86272-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86272-1-RR (same clone as 86272-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FNDC3B antibody (86272-1-RR, Clone: 250827H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86272-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02305-1

  The mean FNDC3B concentration was determined to be 70.0 ng/mL in HepG2 cell extract based on a 0.9 mg/mL extract load.

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  Cytometric bead array standard curve of MP02305-1

  Cytometric bead array standard curve of MP02305-1, FNDC3B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86272-2-PBS. Detection antibody: 86272-1-PBS. Standard: Ag18357. Range: 3.125-200 ng/mL.

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Informations sur le produit

86272-1-PBS cible FNDC3B dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FNDC3B Protéine recombinante Ag18357
• Nom complet: fibronectin type III domain containing 3B
• Masse moléculaire calculée: 1204 aa, 133 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC039297
• Symbole du gène: FNDC3B
• Identification du gène (NCBI): 64778
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Fibronectin type III domain-containing protein 3B(FNDC3B), also named FAD104, belongs to the FNDC3 family. NDC3B is a positive regulator of adipocyte differentiation and is expressed at an early stage of adipocyte differentiation. FNDC3B is an endoplasmic reticulum transmembrane protein with a single transmembrane domain at the C terminus preceded by nine repeated fibronectin type III domains(PMID: 32966780). Due to the ability of the fibronectin type III domain to bind to a variety of proteins, FNDC3B plays a crucial role in cell adhesion, proliferation, and growth signaling. FNDC3B is abnormally expressed in a variety of human cancers, including hepatocellular carcinoma, acute myeloid leukemia, colorectal carcinoma, and cervical carcinoma(PMID: 36275754).

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