Anticorps Polyclonal de lapin anti-FNDC5

FNDC5 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(35)

Avis (1)

N° de cat : 23995-1-AP

Synonymes

Irisin, FRCP2, Fibronectin type III repeat-containing protein 2, Fibronectin type III domain-containing protein 5

---

Galerie de données de validation

• 

  WB analysis using 23995-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23995-1-AP (FNDC5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 23995-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23995-1-AP (FNDC5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 23995-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23995-1-AP (FNDC5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 23995-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23995-1-AP (FNDC5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 23995-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23995-1-AP (FNDC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu de muscle squelettique de souris, tissu d'estomac de souris, tissu hépatique de rat
• Résultats positifs en IHC: tissu de muscle squelettique de souris, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 28 publications below
• IHC: See 8 publications below
• IF: See 4 publications below

Informations sur le produit

23995-1-AP cible FNDC5 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FNDC5 Protéine recombinante Ag21195
• Nom complet: fibronectin type III domain containing 5
• Masse moléculaire calculée: 212 aa, 24 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC062297
• Symbole du gène: FNDC5
• Identification du gène (NCBI): 252995
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

fibronectin type III domain containing 5(FNDC5)encodes 23kDa protein, which is a membrane protein that is cleaved and secreted as a newly identified hormone, irisin. The exercise induces muscle FNDC5 expression. Exercise-induced FNDC5 gene expression in muscles was accompanied by a parallel increase in the concentration of circulating irisin, which in turn activates adipocyte thermogenic programs, leading to mitochondrial heat production and energy expenditure.

Publications

Species	Application	Title
	WB	Clin Transl Med; CRISPRa-based activation of Fgf21 and Fndc5 ameliorates obesity by promoting adipocytes browning; Authors - Hongtao Zhu
mouse	WB	Neuropathol Appl Neurobiol; Irisin treatment lowers levels of phosphorylated tau in the hippocampus of pre-symptomatic female but not male htau mice.; Authors - Katie A Bretland
mouse	WB	Br J Pharmacol; PMID: 31339170; Authors - Shengnan Shen
mouse	WB	Aging (Albany NY); Vitellogenin 2 promotes muscle development and stimulates the browning of white fat; Authors - Yilei Li
		J Am Heart Assoc; Irisin Lowers Blood Pressure by Improvement of Endothelial Dysfunction via AMPK-Akt-eNOS-NO Pathway in the Spontaneously Hypertensive Rat.; Authors - Jinjuan Fu
mouse	IHC	Biochim Biophys Acta Mol Basis Dis; Irisin is induced in renal ischemia-reperfusion to protect against tubular cell injury via suppressing p53.; Authors - Yuxue Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Mohammad (Verified Customer) (12-02-2019)

1:600 in mice hippocampusworks well with no non-specific band

• Applications: Western Blot,

{{ptg:RelatedPrimaryAntibodies}}