Anticorps Monoclonal anti-c-Fos

• Phare
• Validé par KD/KO

c-Fos Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(109)

CloneNo.

1G2C5

Avis (2)

N° de cat : 66590-1-Ig

Synonymes

FOS, Fos proto-oncogene, AP-1 transcription factor subunit, Cellular oncogene fos, C FOS, AP 1

---

Galerie de données de validation

• 

  WB analysis using 66590-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66590-1-Ig (c-Fos antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66590-1-Ig

  WB result of c-Fos antibody (66590-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-c-Fos transfected HepG2 cells.

• 

  WB analysis using 66590-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66590-1-Ig (c-Fos antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66590-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66590-1-Ig (c-Fos antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66590-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66590-1-Ig (FOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules RAW 264.7, cellules THP-1, cellules U-937

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 87 publications below
• IHC: See 14 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

66590-1-Ig cible c-Fos dans les applications de WB, IHC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, Lapin, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: c-Fos Protéine recombinante Ag24340
• Nom complet: FOS
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC004490
• Symbole du gène: c-Fos
• Identification du gène (NCBI): 2353
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

c-Fos, also named as FOS and G0/G1 switch regulatory protein 7, is a 380 amino acid protein, which contains 1 bZIP (basic-leucine zipper) domain and belongs to the bZIP family. c-Fos is expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, c-Fos undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the c-Fos protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the c-FOS protein becomes nuclear. c-Fos is a very short-lived intracellular protein, which is very easy to degrade. The calculated molecular weight of c-Fos is 40 kDa, but Phosphorylated c-Fos protein is about 60-65 kDa. It is involved in important cellular events, including cell proliferation, differentiation and survival; genes associated with hypoxia; and angiogenesis; which makes its dysregulation an important factor for cancer development. It can also induce a loss of cell polarity and epithelial-mesenchymal transition, leading to invasive and metastatic growth in mammary epithelial cells. Expression of c-Fos is an indirect marker of neuronal activity because c-Fos is often expressed when neurons fire action potentials. Upregulation of c-Fos mRNA in a neuron indicates recent activity.

Publications

Species	Application	Title
mouse		Adv Mater; Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment; Authors - Yuewen Zhai
mouse		Curr Biol; Neural processing of the reward value of pleasant odorants.; Authors - Maëllie Midroit
human	WB	Theranostics; KDM6A promotes imatinib resistance through YY1-mediated transcriptional upregulation of TRKA independently of its demethylase activity in chronic myelogenous leukemia.; Authors - Chengwan Zhang ; KD Validated
mouse		Phytomedicine; Withaferin A protects against epilepsy by promoting LCN2-mediated astrocyte polarization to stopping neuronal ferroptosis; Authors - Zixian Zhou
mouse		Phytomedicine; Gastrodin alleviates NTG-induced migraine-like pain via inhibiting succinate/HIF-1α/TRPM2 signaling pathway in trigeminal ganglion; Authors - Chao Ma
mouse	WB	J Headache Pain; SS-31 alleviated nociceptive responses and restored mitochondrial function in a headache mouse model via Sirt3/Pgc-1α positive feedback loop; Authors - Zhengming Shan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Reyes (Verified Customer) (02-14-2025)

cFOS (a nuclear marker) did not perform as expected in my epileptic human FFPE tissue. It seems to appear in the nucleus in a few cells, but also a strong marking in the cyoplasm of others.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

c-Fos Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:66590-1-Ig)

FH

Tatyana (Verified Customer) (01-21-2023)

Suitable for IHC in the paraffinised brain sections of mice (cortex). Samples were fixed in 4% and standard IHC procedure with antigen retrieval and DAB detection was performed. Antibody was incubated at 1:1000 dilution overnight at 4C. Provided a good specific signal in neurons that increased after restraint stress.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse CNS tissue

c-Fos Antibody Immunohistochemistry validation (1:1000 dilution) in Mouse CNS tissue (Cat no:66590-1-Ig)

{{ptg:RelatedPrimaryAntibodies}}