Anticorps Monoclonal anti-c-Fos

• Phare
• Validé par KD/KO

c-Fos Monoclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

56

CloneNo.

1G2C5

Avis (1)

N° de cat : 66590-1-Ig

Synonymes

AP 1, C FOS, Cellular oncogene fos, c-Fos, FOS, G0S7, Proto oncogene c Fos

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Galerie de données de validation

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  WB analysis of HepG2 using 66590-1-Ig

  WB result of c-Fos antibody (66590-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-c-Fos transfected HepG2 cells.

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  WB analysis using 66590-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66590-1-Ig (c-Fos antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66590-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66590-1-Ig (c-Fos antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66590-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66590-1-Ig (c-Fos antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66590-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66590-1-Ig (FOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 66590-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human c-Fos (66590-1-Ig, Clone:1G2C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules RAW 264.7, cellules THP-1, cellules U-937
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 48 publications below
• IHC: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

66590-1-Ig cible c-Fos dans les applications de WB, IP, IHC, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: c-Fos Protéine recombinante Ag24340
• Nom complet: FOS
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC004490
• Symbole du gène: FOS
• Identification du gène (NCBI): 2353
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

c-Fos, also named as FOS and G0/G1 switch regulatory protein 7, is a 380 amino acid protein, which contains 1 bZIP (basic-leucine zipper) domain and belongs to the bZIP family. c-Fos is expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, c-Fos undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the c-Fos protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the c-FOS protein becomes nuclear. c-Fos is a very short-lived intracellular protein, which is very easy to degrade. The calculated molecular weight of c-Fos is 40 kDa, but Phosphorylated c-Fos protein is about 60-65 kDa. It is involved in important cellular events, including cell proliferation, differentiation and survival; genes associated with hypoxia; and angiogenesis; which makes its dysregulation an important factor for cancer development. It can also induce a loss of cell polarity and epithelial-mesenchymal transition, leading to invasive and metastatic growth in mammary epithelial cells. Expression of c-Fos is an indirect marker of neuronal activity because c-Fos is often expressed when neurons fire action potentials. Upregulation of c-Fos mRNA in a neuron indicates recent activity.

Publications

Species	Application	Title
mouse		Curr Biol; Neural processing of the reward value of pleasant odorants.; Authors - Maëllie Midroit
human	WB	Theranostics; KDM6A promotes imatinib resistance through YY1-mediated transcriptional upregulation of TRKA independently of its demethylase activity in chronic myelogenous leukemia.; Authors - Chengwan Zhang ; KD Validated
mouse	WB	J Headache Pain; SS-31 alleviated nociceptive responses and restored mitochondrial function in a headache mouse model via Sirt3/Pgc-1α positive feedback loop; Authors - Zhengming Shan
	WB	J Headache Pain; PACAP6-38 improves nitroglycerin-induced central sensitization by modulating synaptic plasticity at the trigeminal nucleus caudalis in a male rat model of chronic migraine; Authors - Lily Zhang
human	WB	Cancer Lett; MicroRNA-490-3P targets CDK1 and inhibits ovarian epithelial carcinoma tumorigenesis and progression.; Authors - Shuo Chen
mouse	WB	J Nanobiotechnology; The ketone body β-hydroxybutyrate alleviates CoCrMo alloy particles induced osteolysis by regulating NLRP3 inflammasome and osteoclast differentiation.; Authors - Yanglin Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tatyana (Verified Customer) (01-21-2023)

Suitable for IHC in the paraffinised brain sections of mice (cortex). Samples were fixed in 4% and standard IHC procedure with antigen retrieval and DAB detection was performed. Antibody was incubated at 1:1000 dilution overnight at 4C. Provided a good specific signal in neurons that increased after restraint stress.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse CNS tissue

c-Fos Antibody Immunohistochemistry validation (1:1000 dilution) in Mouse CNS tissue (Cat no:66590-1-Ig)