Anticorps Polyclonal de lapin anti-FOXA2

• Phare
• Validé par KD/KO

FOXA2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF-P, IP, CoIP, chIP, ELISA

Conjugaison

Non conjugué

Publications(26)

Avis (3)

N° de cat : 22474-1-AP

Synonymes

forkhead box A2, Forkhead box protein A2, Hepatocyte nuclear factor 3-beta, HNF 3 beta, HNF 3B

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Galerie de données de validation

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  WB analysis using 22474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22474-1-AP (FOXA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 22474-1-AP

  WB result of FOXA2 antibody (22474-1-AP; 1:600; room temperature for 1.5 hours) with wild-type and sg-FOXA2 transfected HepG2 cells.

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  IP experiment of HepG2 using 22474-1-AP

  IP result of anti-FOXA2 (IP:22474-1-AP, 4ug; Detection:22474-1-AP 1:500) with HepG2 cells lysate 1320 ug.

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  IHC staining of human colon cancer using 22474-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22474-1-AP (FOXA2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 22474-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22474-1-AP (FOXA2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat stomach using 22474-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat stomach tissue using FOXA2 antibody (22474-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules A549, cellules HepG2, cellules MDA-MB-231
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'estomac de rat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

22474-1-AP cible FOXA2 dans les applications de WB, IHC, IF-P, IP, CoIP, chIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FOXA2 Protéine recombinante Ag18221
• Nom complet: forkhead box A2
• Masse moléculaire calculée: 457 aa, 48 kDa
• Poids moléculaire observé: 55-65 kDa
• Numéro d’acquisition GenBank: BC011780
• Symbole du gène: FOXA2
• Identification du gène (NCBI): 3170
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FOXA2 also named as HNF 3 beta or TCF3B is a 457 amino acid protein, which contains one fork-head DNA-binding domains. FOXA2 can Shuttle between the nucleus and cytoplasm in a CRM1-dependent manner. Phosphorylation on FOXA2 Thr-156 abolishes binding to target promoters and subsequent transcription activation upon INS stimulation. FOXA2 as a transcription factor involves in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. The molecular weight of FOXA2 is 48kDa, but the phosphorylated FOXA2 may be a 55-65kDa protein.

Publications

Species	Application	Title
human	chIP	Cell Res; Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer.; Authors - Shuaishuai Teng
human	WB	iScience; NatD epigenetically activates FOXA2 expression to promote breast cancer progression by facilitating MMP14 expression; Authors - Mengying Xing
human	IF	Chemosphere; Comparative cytotoxicity studies of halophenolic disinfection byproducts using human extended pluripotent stem cells.; Authors - Yujie Liu
human	WB,IHC,IF	Am J Cancer Res; Epigenetically silenced linc00261 contributes to the metastasis of hepatocellular carcinoma via inducing the deficiency of FOXA2 transcription.; Authors - Zhanjun Chen ; KD Validated
human	WB	Int J Biol Sci; EGF suppresses the expression of miR-124a in pancreatic β cell lines via ETS2 activation through the MEK and PI3K signaling pathways.; Authors - Lin Yang
mouse	WB	Mol Cell Endocrinol; Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells.; Authors - Xinghong Guo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Gökçe Nihan (Verified Customer) (10-02-2025)

Immunofluorescence and Western blot applications worked beautifully with this antibody for both mouse and human samples.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:200 IF, 1:2500 WB
• Cell Tissue Type: hiPSC derived neuroectodermal cells and mouse primary NPCs

FH

Kamal (Verified Customer) (07-05-2023)

Worked when diluted in TBST for WB and 1X PBS for IFC.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000 (WB) and 1:200 (IFC)
• Cell Tissue Type: Mouse Liver tissue

FH

Kamal (Verified Customer) (07-05-2023)

Worked when diluted in TBST for WB and 1X PBS for IFC.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000 (WB) and 1:200 (IFC)
• Cell Tissue Type: Mouse Liver tissue

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