Anticorps Recombinant de lapin anti-FOXK2

• Phare
• Validé par KD/KO

FOXK2 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

240692A5

N° de cat : 83735-4-RR

Synonymes

240692A5, forkhead box K2, Forkhead box protein K2, ILF, ILF 1

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Galerie de données de validation

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  WB analysis of HAP1 using 83735-4-RR

  WB result of FOXK2 antibody (83735-4-RR; 1:20000; room temperature for 1.5 hours) with wild-type and sg-FOXK2 transfected HAP1 cells.

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  WB analysis using 83735-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83735-4-RR (FOXK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 83735-4-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83735-4-RR (FOXK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 83735-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using FOXK2 antibody (83735-4-RR, Clone: 240692A5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A431 using 83735-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using FOXK2 antibody (83735-4-RR, Clone: 240692A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of SH-SY5Y using 83735-4-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug FOXK2 Recombinant antibody (83735-4-RR, Clone:240692A5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of HEK-293T using 83735-4-RR

  1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug FOXK2 Recombinant antibody (83735-4-RR, Clone:240692A5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  ChIP experiment of HepG2 using 83735-4-RR

  Chromatin was prepared from HepG2 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of FOXK2 (83735-4-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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  Affinity and Kinetic Characterization of 83735-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83735-4-RR against Human FOXK2 were performed. The affinity constant is 0.119 nM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules MCF-7, cellules MDA-MB-231, cellules T-47D, cellules U2OS
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,
• Résultats positifs en FC (Intra): cellules SH-SY5Y, cellules HEK-293T
• Résultats positifs en ChIP-qPCR: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83735-4-RR cible FOXK2 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FOXK2 Protéine recombinante Ag33625
• Nom complet: forkhead box K2
• Masse moléculaire calculée: 69 kDa
• Poids moléculaire observé: 69-79 kDa
• Numéro d’acquisition GenBank: NM_004514.3
• Symbole du gène: FOXK2
• Identification du gène (NCBI): 3607
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FOXK2 (forkhead box K2), also known as ILF and ILF1. FOXK2 is localized at nucleus and cytoplasm. It is also expected to be expressed in testis, brain and lymphoid and non-lymphoid cells (PMID: 1339390). The protein encoded by this gene contains a fork head DNA binding domain. This protein can bind to the purine-rich motifs of the HIV long terminal repeat (LTR), and to the similar purine-rich motif in the interleukin 2 (IL2) promoter. It may be involved in the regulation of viral and cellular promoter elements. And its molecular weight is 69 kDa. Anett Marais demonstrate that FOXK2 is phosphorylated in a cell cycle-dependent manner. This phosphorylation peaks during M phase and is mediated by CDK·cyclin complexes. FOXK2 exhibits a periodic rise in its phosphorylation levels during the cell cycle, with hyperphosphorylation occurring in mitotic cells (PMID: 20810654).

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