Anticorps Recombinant de lapin anti-FOXK2

FOXK2 Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240692D6

N° de cat : 83735-5-RR

Synonymes

ILF1, ILF 1, ILF, Forkhead box protein K2, forkhead box K2

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Galerie de données de validation

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  IHC staining of human placenta using 83735-5-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83735-5-RR (FOXK2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 83735-5-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83735-5-RR (FOXK2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 83735-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 83735-5-RR (FOXK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 83735-5-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83735-5-RR (FOXK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 83735-5-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using FOXK2 antibody (83735-5-RR, Clone: 240692D6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A431 using 83735-5-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using FOXK2 antibody (83735-5-RR, Clone: 240692D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of SH-SY5Y using 83735-5-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Foxk2 Recombinant Antibody (83735-5-RR, Clone:240692D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of HEK-293T using 83735-5-RR

  1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug Foxk2 Recombinant Antibody (83735-5-RR, Clone:240692D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IHC: tissu placentaire humain, tissu de cancer de l'estomac humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,
• Résultats positifs en FC (Intra): cellules HEK-293T, cellules SH-SY5Y

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83735-5-RR cible FOXK2 dans les applications de IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FOXK2 Protéine recombinante Ag33625
• Nom complet: forkhead box K2
• Masse moléculaire calculée: 69 kDa
• Numéro d’acquisition GenBank: NM_004514.3
• Symbole du gène: FOXK2
• Identification du gène (NCBI): 3607
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

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