Anticorps Recombinant de lapin anti-FOXM1

FOXM1 Recombinant Antibody for WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

242077A7

N° de cat : 84681-1-PBS

Synonymes

242077A7, FKHL16, Forkhead-related protein FKHL16, HFH11, HFH-11

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Galerie de données de validation

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  WB analysis using 84681-1-RR (same clone as 84681-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 84681-1-RR (FOXM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84681-1-PBS in a different storage buffer formulation.

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  WB analysis using 84681-1-RR (same clone as 84681-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 84681-1-RR (FOXM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84681-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01500-3

  Sandwich ELISA standard curve of MP01500-3, Human FOXM1 Recombinant Matched Antibody Pair - PBS only. 84681-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3729. 84681-1-PBS was HRP conjugated as the detection antibody. Range: 156-5000 pg/mL.

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  Sample test of MP01500-3

  The mean FOXM1 concentration was determined to be 3.3 ng/mL in NCI-H1299 cell extract based on a 1.5 mg/mL extract load.

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  FC experiment of HeLa using 84681-1-RR (same clone as 84681-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug FOXM1 Recombinant antibody (84681-1-RR, Clone:242077A7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84681-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 84681-1-RR (same clone as 84681-1-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug FOXM1 Recombinant antibody (84681-1-RR, Clone:242077A7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84681-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84681-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84681-1-RR against Human FOXM1 were performed. The affinity constant is 10.7 pM.

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Informations sur le produit

84681-1-PBS cible FOXM1 dans les applications de WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FOXM1 Protéine recombinante Ag3729
• Nom complet: forkhead box M 1
• Masse moléculaire calculée: 83 kDa
• Poids moléculaire observé: 84-110 kDa
• Numéro d’acquisition GenBank: BC006192
• Symbole du gène: FOXM1
• Identification du gène (NCBI): 2305
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Forkhead box M1 (FOXM1), also named as HNF-3, HFH-11 or Trident, belongs to a superfamily of Fox transcription factors. FOXM1 is strongly as5sociated with cell proliferation, it plays a important role in cell cycle progression by regulating both G1/S and G2/M phases, it is also involved in DNA damage repair, angiogenesis, apoptosis and tissue regeneration. Recently, crucial roles of this transcription factor were found in the development and progression of many cancers including colorectal, lung, prostate, liver and breast cancer. FOXM1 levels are significantly higher and associated with tumor grade in most human tumors, rendering it a potential target of cancer diagnosis and therapies. Catalog#13147-1-AP is a rabbit polyclonal antibody raised against C-terminal of human FOXM1. And this antibody can recognize three kinds of FOXM1, FOXM1-pp(~95-110kDa), FOXM1-B(isoform2,~83kDa) and FOXM1A(isoform4, ~90kDa). Sometimes a molecular weight of about 65 kDa can also be observed, which may be a degradation form of FOXM1 (PMID: 26547933).

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