Anticorps Recombinant de lapin anti-FOXM1

FOXM1 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

242077A7

N° de cat : 84681-1-RR

Synonymes

HNF-3/fork-head homolog 11, HFH-11, HFH11, Forkhead-related protein FKHL16, FKHL16

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Galerie de données de validation

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  WB analysis using 84681-1-RR

  A431 cells were subjected to SDS PAGE followed by western blot with 84681-1-RR (FOXM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84681-1-RR

  L02 cells were subjected to SDS PAGE followed by western blot with 84681-1-RR (FOXM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 84681-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug FOXM1 Recombinant antibody (84681-1-RR, Clone:242077A7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  FC experiment of A549 using 84681-1-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug FOXM1 Recombinant antibody (84681-1-RR, Clone:242077A7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 84681-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84681-1-RR against Human FOXM1 were performed. The affinity constant is 10.7 pM.

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Applications testées

• Résultats positifs en WB: cellules L02, cellules A431, cellules HT-29, cellules U2OS
• Résultats positifs en FC (Intra): cellules A549, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84681-1-RR cible FOXM1 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FOXM1 Protéine recombinante Ag3729
• Nom complet: forkhead box M 1
• Masse moléculaire calculée: 83 kDa
• Poids moléculaire observé: 84-110 kDa
• Numéro d’acquisition GenBank: BC006192
• Symbole du gène: FOXM1
• Identification du gène (NCBI): 2305
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Forkhead box M1 (FOXM1), also named as HNF-3, HFH-11 or Trident, belongs to a superfamily of Fox transcription factors. FOXM1 is strongly as5sociated with cell proliferation, it plays a important role in cell cycle progression by regulating both G1/S and G2/M phases, it is also involved in DNA damage repair, angiogenesis, apoptosis and tissue regeneration. Recently, crucial roles of this transcription factor were found in the development and progression of many cancers including colorectal, lung, prostate, liver and breast cancer. FOXM1 levels are significantly higher and associated with tumor grade in most human tumors, rendering it a potential target of cancer diagnosis and therapies. Catalog#13147-1-AP is a rabbit polyclonal antibody raised against C-terminal of human FOXM1. And this antibody can recognize three kinds of FOXM1, FOXM1-pp(~95-110kDa), FOXM1-B(isoform2,~83kDa) and FOXM1A(isoform4, ~90kDa). Sometimes a molecular weight of about 65 kDa can also be observed, which may be a degradation form of FOXM1 (PMID: 26547933).

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