Anticorps Monoclonal anti-FOXN1

FOXN1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D8B12

N° de cat : 66337-1-PBS

Synonymes

Winged-helix transcription factor nude, WHN, RONU, Forkhead box protein N1, forkhead box N1

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Galerie de données de validation

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  WB analysis of Raji using 66337-1-Ig (same clone as 66337-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66337-1-Ig (FOXN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66337-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66337-1-Ig (same clone as 66337-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66337-1-Ig (FOXN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66337-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse thymus using 66337-1-Ig (same clone as 66337-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 66337-1-Ig (FOXN1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66337-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse thymus using 66337-1-Ig (same clone as 66337-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 66337-1-Ig (FOXN1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66337-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 66337-1-Ig (same clone as 66337-1-PBS)

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human FOXN1 (66337-1-Ig, Clone:1D8B12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 66337-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66337-1-PBS cible FOXN1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FOXN1 Protéine recombinante Ag18207
• Nom complet: forkhead box N1
• Masse moléculaire calculée: 648 aa, 69 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC146539
• Symbole du gène: FOXN1
• Identification du gène (NCBI): 8456
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FOXN1, also named as Forkhead box protein N1, is a 648 amino acid protein, which contains 1 fork-head DNA-binding domain and localizes in the nucleus. FOXN1 as transcriptional regulator regulates the development, differentiation, and function of thymic epithelial cells (TECs) both in the prenatal and postnatal thymus. FOXN1 acts as a master regulator of the TECs lineage development and is required from the onset of differentiation in progenitor TECs in the developing fetus to the final differentiation steps through which TECs mature to acquire their full functionality.

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