Anticorps Monoclonal anti-FOXO1

• Phare
• Validé par KD/KO

FOXO1 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications(12)

CloneNo.

1D9A2

Avis (1)

N° de cat : 66457-1-Ig

Synonymes

1D9A2, FKH 1, FKH1, FKHR, forkhead box O1

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Galerie de données de validation

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  WB analysis using 66457-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66457-1-Ig (FOXO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66457-1-Ig

  WB result of FOXO1 antibody (66457-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXO1 transfected HEK-293 cells.

• 

  WB analysis of HeLa using 66457-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66457-1-Ig (FOXO1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 66457-1-Ig

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 66457-1-Ig (FOXO1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 66457-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66457-1-Ig (FOXO1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 66457-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66457-1-Ig (FOXO1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules L02, cellules SMMC-7721
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

66457-1-Ig cible FOXO1 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Chèvre, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FOXO1 Protéine recombinante Ag13296
• Nom complet: forkhead box O1
• Masse moléculaire calculée: 655 aa, 70 kDa
• Poids moléculaire observé: 68-70 kDa
• Numéro d’acquisition GenBank: BC021981
• Symbole du gène: FOXO1
• Identification du gène (NCBI): 2308
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).

Publications

Species	Application	Title
human	WB	Transl Psychiatry; Psychosocial and behavioral problems of children and adolescents in the early stage of reopening schools after the COVID-19 pandemic: a national cross-sectional study in China.; Authors - Lin Wang
human	WB	Int J Biol Sci; ATIC inhibits autophagy in hepatocellular cancer through the AKT/FOXO3 pathway and serves as a prognostic signature for modeling patient survival.; Authors - Hao Zhang
human	WB	Int Immunopharmacol; 1β-Hydroxyalantolactone from Inulae Flos alleviated the progression of pulmonary fibrosis via inhibiting JNK/FOXO1/NF-κB pathway; Authors - Bing Yu
human	WB	Thorac Cancer; Serum exosomal miR-1269a serves as a diagnostic marker and plays an oncogenic role in non-small cell lung cancer.; Authors - Xue Wang ; KD Validated
	WB	Equine Vet J; Muscle fibre transition and transcriptional changes of horse skeletal muscles during traditional Mongolian endurance training; Authors - Tugeqin Bou
mouse	WB	Ecotoxicol Environ Saf; Integrated network toxicology, molecular docking, and in vivo experiments to elucidate molecular mechanism of aflatoxin B1 hepatotoxicity; Authors - Bingjie Ge

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Refik (Verified Customer) (09-07-2025)

Works well with overnight incubation with 1:250 in 1x PBST with 5% BSA for ICC staining of human-derived cells with 3 hours at RTP with 1:400 secondary antibody in 1x PBST with 5% Donkey Serum and DAPI

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:250 for ICC staining of human-derived cells with 1:400 secondary antibody
• Cell Tissue Type: Human-derived cardiac cells

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