Anticorps Monoclonal anti-FOXO1

• Phare
• Validé par KD/KO

FOXO1 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1D9A2

N° de cat : 66457-1-PBS

Synonymes

1D9A2, FKH 1, FKH1, FKHR, forkhead box O1

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Galerie de données de validation

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  WB analysis using 66457-1-Ig (same clone as 66457-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66457-1-Ig (FOXO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66457-1-Ig (same clone as 66457-1-PBS)

  WB result of FOXO1 antibody (66457-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXO1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66457-1-Ig (same clone as 66457-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66457-1-Ig (FOXO1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

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  WB analysis of SMMC-7721 using 66457-1-Ig (same clone as 66457-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 66457-1-Ig (FOXO1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66457-1-Ig (same clone as 66457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66457-1-Ig (FOXO1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66457-1-Ig (same clone as 66457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66457-1-Ig (FOXO1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66457-1-PBS cible FOXO1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FOXO1 Protéine recombinante Ag13296
• Nom complet: forkhead box O1
• Masse moléculaire calculée: 655 aa, 70 kDa
• Poids moléculaire observé: 68-70 kDa
• Numéro d’acquisition GenBank: BC021981
• Symbole du gène: FOXO1
• Identification du gène (NCBI): 2308
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).

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