Anticorps Recombinant de lapin anti-FOXO1

FOXO1 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

230183D1

N° de cat : 82997-1-RR

Synonymes

Forkhead box protein O1, forkhead box O1, FKHR, FKH1, FKH 1

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Galerie de données de validation

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  WB analysis using 82997-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82997-1-RR (FOXO1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 82997-1-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 82997-1-RR (FOXO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 82997-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FOXO1 antibody (82997-1-RR, Clone: 230183D1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  FC experiment of A431 using 82997-1-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug FOXO1 Recombinant antibody (82997-1-RR, Clone:230183D1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 82997-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82997-1-RR against Human FOXO1 were performed. The affinity constant is 0.188 nM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HepG2, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

82997-1-RR cible FOXO1 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FOXO1 Protéine recombinante Ag13296
• Nom complet: forkhead box O1
• Masse moléculaire calculée: 655 aa, 70 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC021981
• Symbole du gène: FOXO1
• Identification du gène (NCBI): 2308
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).

Publications

Species	Application	Title
human	WB	J Ethnopharmacol; Chinese herbal medicine alleviates autophagy and apoptosis in ovarian granulosa cells induced by testosterone through PI3K/AKT1/FOXO1 pathway; Authors - Weihuan Hu

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