Anticorps Recombinant de lapin anti-FOXO1

FOXO1 Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230183G9

N° de cat : 82997-2-PBS

Synonymes

FKH 1, FKH1, FKHR, forkhead box O1, Forkhead box protein O1

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Galerie de données de validation

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  Sandwich ELISA sample test of MP00024-3

  Serum of sixteen individual healthy human donors was measured. The human FOXO1 concentration of detected samples was determined to be 359.1 pg/mL with a range of 18.5 - 4,538.3 pg/mL.

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  Sandwich ELISA sample test of MP00024-3

  MCF-7, HEK-293T and C2C12 cell lysates were measured. The human FOXO1 concentration of detected samples was determined to be 416.1 pg/mL (based on a 1.2 mg/mL extract load) in MCF-7 cell lysate, 637.3 pg/mL (based on a 1.4 mg/mL extract load) in HEK-293T cell lysate and 1,050.2 pg/mL (based on a 3.2 mg/mL extract load) in C2C12 cell lysate.

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  Sandwich ELISA standard curve of MP00024-3

  Sandwich ELISA standard curve of MP00024-3, Human FOXO1 Recombinant Matched Antibody Pair - PBS only. 82997-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34671. 82997-5-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL

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  Affinity and Kinetic Characterization of 82997-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 82997-2-PBS against Human FOXO1 was performed. The affinity constant is 0.603 nM.

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Informations sur le produit

82997-2-PBS cible FOXO1 dans les applications de Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FOXO1 Protéine recombinante Ag13296
• Nom complet: forkhead box O1
• Masse moléculaire calculée: 655 aa, 70 kDa
• Numéro d’acquisition GenBank: BC021981
• Symbole du gène: FOXO1
• Identification du gène (NCBI): 2308
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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