Anticorps Polyclonal de lapin anti-FOXO3A

• Phare
• Validé par KD/KO

FOXO3A Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(135)

Avis (3)

N° de cat : 10849-1-AP

Synonymes

FOXO3, AF6q21, AF6q21 protein, FKHRL1, FKHRL1P2

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Galerie de données de validation

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  WB analysis of HEK-293 using 10849-1-AP

  WB result of FOXO3A antibody (10849-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXO3A transfected HEK-293 cells.

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  WB analysis using 10849-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10849-1-AP (FOXO3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10849-1-AP

  IP result of anti-FOXO3A (IP:10849-1-AP, 4ug; Detection:10849-1-AP 1:10000) with HEK-293 cells lysate 1600 ug.

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  IHC staining of human colon cancer using 10849-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10849-1-AP (FOXO3A antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10849-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10849-1-AP (FOXO3A antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Jurkat, cellules MCF-7, cellules Neuro-2a, cellules PC-3
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:3000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 13 publications below
• WB: See 122 publications below
• IHC: See 15 publications below
• IF: See 20 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

10849-1-AP cible FOXO3A dans les applications de WB, IHC, IF, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FOXO3A Protéine recombinante Ag1289
• Nom complet: forkhead box O3
• Masse moléculaire calculée: 673 aa, 71 kDa
• Poids moléculaire observé: 72-97 kDa
• Numéro d’acquisition GenBank: BC021224
• Symbole du gène: FOXO3A
• Identification du gène (NCBI): 2309
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

In mammalian cells, FoxO factors act as potent transcriptional activators that upregulate the expression of programs of genes involved in stress resistance, cell cycle arrest, differentiation, apoptosis, autophagy, and metabolism. FOXO3 has a key role in the maintenance of adult neural and hematopoietic stem cells. It also associates with extreme longevity in humans and regulates the homeostasis of adult stem cell pools in mammals, which may contribute to longevity. Phosphorylation inhibits FOXO3 nuclear translocation that is essential for its regulation and function. The calculated molecular weight of FOXO3 is 71 kDa,but some experiments analysis using a FOXO3a specific antibody showed the expression of a 90 kDa fulllength FOXO3a protein (Full) and a 40 kDa processed FOXO3a product (Short). 90 kDa FOXO3a protein is a phosphorylated via activation of PI3K (phosphatidylinositol 3-kinase) and Akt after INS stimulation, and translocate from the nucleus to the cytoplasm. (PMID: 19427386)

Publications

Species	Application	Title
mouse,human	WB,IP	J Hazard Mater; Low-dose deoxynivalenol exposure inhibits hepatic mitophagy and hesperidin reverses this phenomenon by activating SIRT1; Authors - Hao Chen
human	WB	Nat Commun; PRMT5-mediated arginine methylation activates AKT kinase to govern tumorigenesis.; Authors - Shasha Yin
human	WB	Nat Commun; Small extracellular vesicle-mediated miR-320e transmission promotes osteogenesis in OPLL by targeting TAK1.; Authors - Chen Xu
mouse	WB	Aging Cell; Activation of angiotensin-converting enzyme 2/angiotensin (1-7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling; Authors - Ruili Dang
mouse	WB	J Adv Res; Core fucosylation regulates the ovarian response via FSH receptor during follicular development; Authors - Tiantong Wang
rat,mouse	WB	J Nanobiotechnology; Dental pulp mesenchymal stem cell-derived exosomes inhibit neuroinflammation and microglial pyroptosis in subarachnoid hemorrhage via the miRNA-197-3p/FOXO3 axis; Authors - Xin Liang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Simona (Verified Customer) (03-03-2023)

The FOXO3A band is clearly visible in SH-SY5Y neuroblastoma cells, the last line, consisting of PC3 cells was considered as positive control but the signal was lower than SH-SY5Y. The product is useful.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SH-SY5Y

FOXO3A Antibody Western Blot validation (1:1000 dilution) in SH-SY5Y (Cat no:10849-1-AP)

FH

Valeria (Verified Customer) (11-11-2020)

Fixative: 4% paraformaldehydeAntigen retrieval step: NoPermeabilization: Methanol gradient (70% for 10 mins, 95% for 10 mins, 100% for 20 mins, 95% for 10 mins, 70% for 10 mins)Protein blocking: 10% NGS diluted in TBSTEndogenous biotin blocking: Avidin D solution for 15 mins, Biotin for 15 mins (Vector Laboratories, Inc)Secondary antibody: Life tech/ThermoFisher, A11006, Alexa 488, Goat anti-Rabbit isotype IgG, 1:100

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Human skeletal muscle sections (10um)

FOXO3A Antibody Immunofluorescence validation (1:50 dilution) in Human skeletal muscle sections (10um) (Cat no:10849-1-AP)

FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

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