Anticorps Polyclonal de lapin anti-FOXO4

• Phare
• Validé par KD/KO

FOXO4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications(23)

Avis (1)

N° de cat : 21535-1-AP

Synonymes

AFX, AFX1, forkhead box O4, Forkhead box protein O4, FOXO4, MLLT7

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Galerie de données de validation

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  WB analysis using 21535-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21535-1-AP (FOXO4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 21535-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21535-1-AP (FOXO4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 21535-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21535-1-AP (FOXO4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 21535-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21535-1-AP (FOXO4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules PC-12, tissu cardiaque de souris, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 20 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

21535-1-AP cible FOXO4 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FOXO4 Protéine recombinante Ag16169
• Nom complet: forkhead box O4
• Masse moléculaire calculée: 505 aa, 54 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC106761
• Symbole du gène: FOXO4
• Identification du gène (NCBI): 4303
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FOXO4, also named as AFX, AFX1 and MLLT7, is a transcription factor which involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1.FOXO down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is involved in negative regulation of the cell cycle. A chromosomal aberration involving FOXO4 is found in acute leukemias. The antibody can recognize isoform 1 and isoform Zeta of FOXO4.

Publications

Species	Application	Title
canine	WB	Circ Res; Suppression of Activated FOXO Transcription Factors in the Heart Prolongs Survival in a Mouse Model of Laminopathies.; Authors - Gaelle Auguste
human	WB	J Clin Invest; Hepatitis B virus X protein represses miRNA-148a to enhance tumorigenesis.; Authors - Xu Xiaojie X ; KD Validated
mouse,rat	WB,IHC	Nat Commun; Damaged brain accelerates bone healing by releasing small extracellular vesicles that target osteoprogenitors.; Authors - Wei Xia
rat	WB	Redox Biol; Notoginsenoside R1-loaded mesoporous silica nanoparticles targeting the site of injury through inflammatory cells improves heart repair after myocardial infarction.; Authors - Han Li
human	IF	Mater Today Bio; Ultra-small polydopamine nanomedicine-enabled antioxidation against senescence; Authors - Jiamei Han
rat	WB	J Agric Food Chem; Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.; Authors - Shu Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

zhiping (Verified Customer) (06-04-2021)

It works pretty well for IHC.

• Applications: Immunohistochemistry
• Cell Tissue Type: human skin

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