Anticorps Polyclonal de lapin anti-FOXO6

• Phare
• Validé par KD/KO

FOXO6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(18)

Avis (1)

N° de cat : 19122-1-AP

Synonymes

forkhead box protein O6

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Galerie de données de validation

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  WB analysis using 19122-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19122-1-AP (FOXO6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19122-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19122-1-AP (FOXO6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 19122-1-AP

  IP result of anti-FOXO6 (IP:19122-1-AP, 3ug; Detection:19122-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 19122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19122-1-AP (FOXO6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19122-1-AP (FOXO6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 19122-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19122-1-AP (FOXO6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 19122-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19122-1-AP (FOXO6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 19122-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19122-1-AP (FOXO6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 19122-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19122-1-AP (FOXO6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 19122-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using FOXO6 antibody (19122-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 19122-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using FOXO6 antibody (19122-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules C2C12, tissu cérébral de souris, tissu pulmonaire de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

19122-1-AP cible FOXO6 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: forkhead box protein O6
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: AI361654
• Symbole du gène: FOXO6
• Identification du gène (NCBI): 100132074
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FOXO6 is a member of the murine forkhead family of transcription factors. These forkhead transcription factors may play a role in maintenance. FOXO6 mRNA is predominantly expressed in the developing brain, indicating that FOXO6 may play an important role in the nervous system. FOXO6 has an isoform of 70 kDa when modified after translation (PMID:23639108).

Publications

Species	Application	Title
mouse	WB	Aging Cell; Activation of angiotensin-converting enzyme 2/angiotensin (1-7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling; Authors - Ruili Dang
human	WB, IF	Elife; Inhibition of mutant EGFR in lung cancer cells triggers SOX2-FOXO6-dependent survival pathways.; Authors - S Michael Rothenberg ; KD Validated
pig	WB,ChIP,CoIP	RNA Biol; Enterotoxigenic Escherichia coli infection promotes enteric defensin expression via FOXO6-METTL3-m6A-GPR161 signalling axis.; Authors - Xin Zong ; KD Validated
human	WB	Oncotarget; Involvement of the FOXO6 transcriptional factor in breast carcinogenesis.; Authors - François Lallemand ; KD Validated
human	IHC	Oncotarget; Elevated FOXO6 expression correlates with progression and prognosis in gastric cancer.; Authors - Jia-Hong Wang
human	IF	Mol Cell Biol; Dormant Pluripotent Cells Emerge during Neural Differentiation of Embryonic Stem Cells in a FoxO3-dependent Manner.; Authors - Megumi Ikeda

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Young-Wook (Verified Customer) (10-31-2017)

This antibody detect easily mouse Foxo1 than mouse Foxo6. There is cross reactivity with Foxo1.

• Applications: Western Blot
• Primary Antibody Dilution: 2000
• Cell Tissue Type: 293T overexpression

FOXO6 Antibody Western Blot validation (2000 dilution) in 293T overexpression (Cat no:19122-1-AP)

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