Anticorps Polyclonal de lapin anti-FOXP2

• Phare
• Validé par KD/KO

FOXP2 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(6)

Avis (1)

N° de cat : 20529-1-AP

Synonymes

CAG repeat protein 44, CAGH44, forkhead box P2, Forkhead box protein P2, TNRC10

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Galerie de données de validation

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  WB analysis using 20529-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20529-1-AP (FOXP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 20529-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20529-1-AP (FOXP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 20529-1-AP

  IP result of anti-FOXP2 (IP:20529-1-AP, 4ug; Detection:20529-1-AP 1:500) with mouse spleen tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules Neuro-2a, tissu cérébral de rat, tissu cérébral de souris, tissu pulmonaire de souris
• Résultats positifs en IP: tissu splénique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

20529-1-AP cible FOXP2 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FOXP2 Protéine recombinante Ag14391
• Nom complet: forkhead box P2
• Masse moléculaire calculée: 80 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC018016
• Symbole du gène: FOXP2
• Identification du gène (NCBI): 93986
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FOXP2, Forkhead box protein P2, is associated with speech and language development (PMID: 24765219). FOXP2 is expressed in the brain, lungs, heart and gut. In the brain, it is widely expressed in sensory, limbic and motor structures(PMID: 10880297, PMID:15685218). Mutations in FOXP2 caused the severe speech and language disorder developmental verbal dyspraxia (PMID: 15877281). FOXP2 was detected 2 isoforms of 70 kDa and 80-82 kDa (PMID: 15056696, PMID: 29360038, PMID: 17999357).

Publications

Species	Application	Title
		Elife; FoxP2 isoforms delineate spatiotemporal transcriptional networks for vocal learning in the zebra finch.; Authors - Zachary Daniel Burkett
human	WB	Mol Ther Nucleic Acids; circ-SHKBP1 Regulates the Angiogenesis of U87 Glioma-Exposed Endothelial Cells through miR-544a/FOXP1 and miR-379/FOXP2 Pathways.; Authors - Qianru He
human	WB,IHC,IF,CoIP	J Cancer; Low expression of FOXP2 predicts poor survival and targets caspase-1 to inhibit cell pyroptosis in colorectal cancer.; Authors - Peng Liao ; KD Validated
rat	IHC	BMC Biol; GnRH-driven FTO-mediated RNA m6A modification promotes gonadotropin synthesis and secretion; Authors - Hao-Qi Wang
rat,human	IHC,IF	Commun Biol; FOXP2 overexpression upregulates LAMA4 expression and thereby alleviates preeclampsia by regulating trophoblast behavior; Authors - Sishi Liu
human	IHC	Cell Rep; The endocytic adaptor AP-2 maintains Purkinje cell function by balancing cerebellar parallel and climbing fiber synapses; Authors - Marianna Tolve

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (10-18-2024)

FOXP2 (in green) worked marking my neurons (in red) in FFPE human brain tissue, although other tests to check the specificity of the layer need to be performed.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

FOXP2 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:20529-1-AP)

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