Anticorps Polyclonal de lapin anti-FOXP3

FOXP3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF-P, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(92)

Avis (1)

N° de cat : 22228-1-AP

Synonymes

AIID, DIETER, forkhead box P3, Forkhead box protein P3, Forkhead box protein P3 41 kDa form

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Galerie de données de validation

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  WB analysis of HEK-293 using 22228-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22228-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 22228-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 22228-1-AP (FOXP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 22228-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22228-1-AP (FOXP3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 22228-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using FOXP3 antibody (22228-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CD3 antibody (60181-1-Ig, Clone: 3F3A1, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A375
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 57 publications below
• IHC: See 27 publications below
• IF: See 16 publications below
• IP: See 2 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Informations sur le produit

22228-1-AP cible FOXP3 dans les applications de WB, IHC, IF-P, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FOXP3 Protéine recombinante Ag17571
• Nom complet: forkhead box P3
• Masse moléculaire calculée: 431 aa, 47 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC113401
• Symbole du gène: FOXP3
• Identification du gène (NCBI): 50943
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. POXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human FOXP3.

Publications

Species	Application	Title
mouse	IF	Acta Pharm Sin B; Self-assembled FeS-based cascade bioreactor with enhanced tumor penetration and synergistic treatments to trigger robust cancer immunotherapy; Authors - Hao Ren
mouse	IF	Cancer Res; Epigenetic regulation of CXCL12 plays a critical role in mediating tumor progression and the immune response in osteosarcoma.; Authors - Binghao Li
mouse	IHC	Biomaterials; LncRNA-targeting bio-scaffold mediates triple immune effects for postoperative colorectal cancer immunotherapy.; Authors - Feng Liu
human	WB	Clin Transl Med; MiRNA-516a promotes bladder cancer metastasis by inhibiting MMP9 protein degradation via the AKT/FOXO3A/SMURF1 axis.; Authors - Yuanyuan Chang
human	WB	J Autoimmun; NF-κB-driven miR-34a impairs Treg/Th17 balance via targeting Foxp3.; Authors - Mengxiao Xie
human	WB	Biomed Pharmacother; Glycyrrhetinic acid inhibits non-small cell lung cancer via promotion of Prdx6- and caspase-3-mediated mitochondrial apoptosis; Authors - Qiuyan Guo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anne-Laure (Verified Customer) (12-07-2021)

non-specific staining

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: brain slices from mouse

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