Anticorps Polyclonal de lapin anti-FOXP3

FOXP3 Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, FC, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

56

Avis (1)

N° de cat : 22228-1-AP

Synonymes

AIID, DIETER, forkhead box P3, Forkhead box protein P3, FOXP3, IPEX, JM2, PIDX, Scurfin, XPID

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Galerie de données de validation

• 

  WB analysis of multi-cells/tissue using 22228-1-AP

  WB results of 22228-1-AP (FOXP3 antibody).

• 

  WB analysis of HeLa using 22228-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22228-1-AP (FOXP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22228-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22228-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of multi-cells using 22228-1-AP

  WB results of anti-22228-1-AP (FOXP3 antibody).

• 

  WB analysis of A375 using 22228-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 22228-1-AP (FOXP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 22228-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22228-1-AP (FOXP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 22228-1-AP

  IP Result of anti-FOXP3 (IP:22228-1-AP, 3ug; Detection:22228-1-AP 1:1000) with HeLa cells lysate 1840ug.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A375, cellules HEK-293, multi-cellules, multi-cellules/tissus, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 35 publications below
• FC: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Informations sur le produit

22228-1-AP cible FOXP3 dans les applications de WB, IP, FC, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FOXP3 Protéine recombinante Ag17571
• Nom complet: forkhead box P3
• Masse moléculaire calculée: 431 aa, 47 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC113401
• Symbole du gène: FOXP3
• Identification du gène (NCBI): 50943
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. POXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human FOXP3.

Publications

Species	Application	Title
mouse		Acta Pharm Sin B; Self-assembled FeS-based cascade bioreactor with enhanced tumor penetration and synergistic treatments to trigger robust cancer immunotherapy; Authors - Hao Ren
mouse		Cancer Res; Epigenetic regulation of CXCL12 plays a critical role in mediating tumor progression and the immune response in osteosarcoma.; Authors - Binghao Li
mouse		Biomaterials; LncRNA-targeting bio-scaffold mediates triple immune effects for postoperative colorectal cancer immunotherapy.; Authors - Feng Liu
human	WB	Clin Transl Med; MiRNA-516a promotes bladder cancer metastasis by inhibiting MMP9 protein degradation via the AKT/FOXO3A/SMURF1 axis.; Authors - Yuanyuan Chang
human	WB	J Autoimmun; NF-κB-driven miR-34a impairs Treg/Th17 balance via targeting Foxp3.; Authors - Mengxiao Xie
rat	WB	Biomed Pharmacother; Kuijieling regulates the differentiation of Treg and Th17 cells to ameliorate experimental colitis in rats.; Authors - Yu Long

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Anne-Laure (Verified Customer) (12-07-2021)

non-specific staining

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: brain slices from mouse

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