Anticorps Monoclonal anti-FOXP3

FOXP3 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A1E7

N° de cat : 68803-2-PBS

Synonymes

2A1E7, AIID, DIETER, forkhead box P3, Forkhead box protein P3

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Galerie de données de validation

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  WB analysis of MJ using 68803-2-Ig (same clone as 68803-2-PBS)

  MJ cells were subjected to SDS PAGE followed by western blot with 68803-2-Ig (FOXP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human rectal cancer using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human rectal cancer using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of MJ using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded MJ cells slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of MJ using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded MJ cells slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50216-1

  Cytometric bead array standard curve of MP50216-1, FOXP3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68803-2-PBS. Detection antibody: 68838-1-PBS. Standard:Ag17571. Range: 0.781-100 ng/mL.

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Informations sur le produit

68803-2-PBS cible FOXP3 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FOXP3 Protéine recombinante Ag23089
• Nom complet: forkhead box P3
• Masse moléculaire calculée: 431 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC113401
• Symbole du gène: FOXP3
• Identification du gène (NCBI): 50943
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FOXP3 is a transcription factor belonging to the Forkhead box (FOX) protein family. It contains a forkhead-winged helix domain that acts as a transcriptional activator or repressor. FOXP3 is the master regulator of regulatory T cells (Tregs), a subset of CD4+ T cells with potent suppressive activity. It plays a critical role in maintaining self-tolerance and preventing autoimmune diseases by controlling the development and function of Tregs. The expression of FOXP3 is quite limited, and the cellular taxa present in the tissue are relatively low. Therefore, if you intend to perform Western blotting (WB), it is advisable to enrich the target cells prior to detection.

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